Subject: helping FlyBase
Hi Karen,
As you know, we are doing some work on your stock list for FlyBase, and I
have a question about the Df(1)RA67 stock:
U-#18350 Df(1)RA67 / FM7, y31d sc8 wa snX2 vOf g4 B1 / y+
Ymal106
Breakpts: ?; 20B-D
Comments:
Do you know where the breakpoint data 'Breakpts: ?; 20B-D' came from, or
what it might mean?
I ask because Michael Costa, then of the Weischaus lab, has published an
allele they call fogRA67 and when asked about whether it may be
Df(1)RA67, he said
>The RA67 allele of fog mentioned in my Cell paper is at least derived from
>the the stock called Df(1)RA67 and quite possibly identical to it. When I
>tested the Df(1)RA67 strain that Eric Wieschaus had in the lab, I found
>that it complemented all mutations tested on both the proximal and distal
>side of fog. Therefore, I started calling it fogRA67 since there was no
>genetic evidence that our allele was a deficiency. I never checked
>cytologically for a deletion in this strain and never found any molecular
>evidence of a deletion. Len Kelly has also found that his stock labelled
>Df(1)RA67 also behaves genetically as a simple fog mutation, and it is not
>clear whether Eric got his allele from Len or the Umea stock center.
So you see it is not QUITE clear that they are the same thing, and we would
like to be sure!
Many thanks for your help,
best wishes,
Rachel
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Rachel Drysdale.
FlyBase (Cambridge),
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Subject:
Dear Rachel,
About stock 18350.Df(1)RA67/ FM7, we received that stock
from George Miklos Canberra October -87, with the
following information Df; 114--EA41-.
I did send a copy to Len Kelly in November-88. He told me
that he was already holding a copy of the strain but it was
not behaving genetically as the original Df(1)RA67. That is
all information I have. We have never been testing the stock.
Best wishes,
Karin