Transposon information for two lines submitted to the Bloomington Stock
Center, stocks and information from Geza Adam, Friedrich Miescher Institute,
Basel, Switzerland.
This is the requested information about the UAS-M2C48C (MAP2c) and
UAS-TAU59A (tau) lines. The MAP2c sequences were from rat (Doll, T.
et al. 1990, Nucl. Acid Res. 18, 361) and the tau (htau23) sequences
were from human (Goedert, M. et al. 1989, Neuron 3, 519-526) source.
Both sequences were cut off from pBluescript vector with NotI and
Bsp 120I restriction endonucleases and built in the NotI site of pUAST
vector. MAP2c and tau constructs were refered to as pUAST-MAP2c and
pUAST-htau23, respectively.
Both constructs were injected into yw/yw strain (w allele was w1118).
The insertion site of the transgene were not determined, in both cases
they are on the second chromosome. Both transgenic lines are viable as
homozygotes and after induction no mutant phenotype was observable.