Concerning Alex Gould's insert P{A92}i71:
We've cloned around 150kb around tsh and the only insert we failed to
localize on this walk (we have about 10 others) was Alex's. However I
cannot guarentee we were 100% critical about these southerns, especially
as this P element has no plasmid rescue element. The beta-gal pattern of
P{A92}i71 is certainly similar, and in some cases identical, to all the
other P{tsh} insertions. Genetically it behaves like the others, in that
over a deletion such as Df(2L)TW161, the proximal leg segments in adults
are affected. Finally we did in situs to localize Alex's insertion; it is
definately in 40A and not 40F. In other words I'm sure its nearby (to tsh)
somewhere!
Second message:
P{A92}71 has been looked over a tsh null. All P elements behave the same for
example over a null like tsh, but the phenotype is weaker than over Df(2L)TW161.
This (I believe) can be explained by the fact that tsh8 has regulatory elements
that can act in trans on the insertion chromosome to make tsh 'more' active.
Df161 does not have these regulatory elements. Molecularly we don't know where P{A92} inserts with respect to tsh, in P{A92}71.