Subject: helping FlyBase
Dear Claude,
I am writing about Anne's paper
\*x FBrf0087230 == Goriely et al., 1996, Development 122(5): 1641--1650
which I am curating for FlyBase.
You mention no cytological location for your D-gsc, but we know from Anne
that 21C5-21C6 is the correct location of D-gsc (or Gsc, as it was named
from its sequence record by FlyBase).
FlyBase has gene entries for 60Mun1 (and 60Mun2) which record (correctly)
their cytological location as being 60C (Dessain and McGinnis, 1993, Adv.
Dev. Biochem. 2: 1--55).
Since 60Mun1 = Gsc, the implication is that the original reported cytology
for 60Mun1 was incorrect due to someone mistaking one end of chromosome 2
for the other. Could you (or Scott) confirm that my interpretation is
correct? Does this also mean that 60Mun2 should have a cytological
location of 21C-21D? The 60Mun1 gene record will be merged with the Gsc
record as a result of your paper, but 60Mun2 will stay separate and I
would like to be sure that FlyBase is reporting its cytological location
accurately.
.
Many thanks for your help,
with best wishes,
Rachel.
\--------------------------------------------------------------
Rachel Drysdale.
FlyBase (Cambridge),
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Subject: Re: helping FlyBase
Dear Rachel,
	I received your message through Claude who asked me to answer
your queries.
Your interpretation of the cytological location of the Mun genes is
correct. It was a mistake made in the 1st report by Dessain & McGinnis.
Both genes map very close to each other, actually on a common genomic
phage, at approximately 10kB of each other. They are both located at 21
C5-6. This is also what Mounou Hahn and Herbert Jackle found and their
paper should be coming soon or is already out in EMBO J. (they only
worked on Gsc though). Claude and me are currently writing a short report
about the other Mun gene, 60Mun2 that we simply call Munster and which is
specifically expressed in Bolwig organs.
I hope these few lines will answer your questions.
Do not hesitate to get in touch with me again if necessary.
Yours Sincerely
		Anne Goriely