Subject: Re: Cyclophylin 1 Dear M- By in situ hybridization Cyp-1 maps to 14B11-12. Using my Cyp-1 mutant and measuring protein levels Dp(1;4)r+,Df(1)81h24b does not cover Cyp-1; Dp(1;4)r+,Df(1)81j23a does cover Cyp-1 (this is also very clear by the cytology. Based on molecular mapping by Jones and Rubin (1990) Df(1)l32 maps proximal to the coding region 5' of the gene but by Western blot and CsA binding this Df may not cover Cyp and would be the closest defining breakpoint. Df(1)E150 covers Cyp-1. The gene is transcribed from proximal to distal. There is a 1.5 Kb intron in the gene between codon 23 and 24 of the 165 aa of the protein. and at least two or (probably) three polyA sites used (0.9, 1.0 and 1.3 Kb)- the Northern published by Stamnes did not resolve the 0.9 and 1 Kb bands but I find the other polyA site in the sequence and Reynolds and Tanouye reported the three transcripts at the last mmm fly meeting in Chicago. The genomic sequence of 10Kb around Cyp-1 is in my thesis but may never have been submitted to Genbank. Jones, K.R. and Rubin, G.M. (1990) Molecular analysis of no-on-transient A, a gene required for normal vision in Drosophila. Neuron 4:711-23 I'll fax a detailed map of 14B and Cyp-1 and a complementation table from my thesis they may help you. Can you reference my thesis? It is on microfilm: 'The Genetic and Biochemical Characterization of the Major Cyclophilin Isoform in Drosophila melanogaster.' Suzanne L. Rutherford, 1995 - Biology UcSD. Madueno et al., 1995, Genetics 139(4): 1631--1647 have Cyp-1 proximal to nonA and it is distal (see the map I sent). Suzanne Suzanne Rutherford Howard Hughes Medical Institute University of Chicago 5841 S. Maryland Ave MC1028 Chicago, IL 60637
