lastname : Freeman givenname : Matthew institution : MRC Laboratory of Molecular Biology author1 : M. Freeman year : 1996 journal : Cell volume : In press FBgu ID : FBgu0000002=20 date_updated: Sep 23 1996 7:47:44:176AM transposon: P(gal4-GMR) - Glass Multimer Reporter vector: pP(CaSpeR-hs) synthetic: 5 copies of glass binding site from Rh1 enhancer. Corresponds to construct 29-2 in Ellis et al. Development (1993) 119, 855 vector_comments: Has some hsp70 promoter sequences: toxic UAS constructs can usually be grown at 18=B0. visualization: UAS-lacZ expression: Expressed very strongly in all cells posterior to=20 morphogenetic furrow. Other expression not examined. insertion_symbol: P(GMR-Gal4) \#12 chromosome: 2 insertion_property: viable insertion_comments: This was the weakest insertion obtained. Most others had rough eyes presumably due to toxicity of massively overexpressed Gal4. This one is wild type as a heterozygote at 25oC and lower. As a homozygote, and at higher temperatures, individuals exhibit non-specific eye defects. date_updated: Sep 23 1996 7:47:44:176AM
