Dear Dr. Golic,
I am a curator working for FlyBase and I am presently curating a paper
(Frise et al., 1996, Proc. Natl. Acad. Sci. USA 93(21): 11925-11932) in
which a UAS-FLP construct obtained from you is used.
I would be really grateful if you could let me know the details of how this
construct was made, for example is the FLP coding region the same as in
your heat shock construct and is the vector used pUAST ? Alternatively is
there a paper in which these details are published?
As this construct is new to FlyBase I would like to get some data from you
.. and not just a secondary publication.
I look forward to hearing from you,
Gillian
Reply to: RE>>FlyBase query to Kelly Beumer
Gillian,
I am sorry it took me so long to get back to you. I was meeting deadlines.
My p UASFLP is marked with ry and is constructed as follows. FLP, with the 2
micron poly A tail was excised from pFV17, the same source as for hsFLP and
inserted into pUAST as an Xba-Sal fragment. It was then lifted as a Bam
fragment and put in an intermediate vector, pHSS6, which allowed excison as
an Xba fragment and insertion into pDM23, a Carnagie vector derivative. I
have a map in Macplasmap, if that would be helpful, that I could forward to
you.
Kelly