Subject: GAL4 construct
Norbert,
FlyBase needs a bit more information concerning the GAL4 construct or
insertion called 'rhlGAL4' in Axelrod, et al., 1996, Science  271:1826 .
Is this an insertion of P{GawB}? A GAL4 construct? If so, with regulatory
sequences from what gene? To what does 'rhl' refer?
Thanks for any enlightenment you can provide!
\--Lynn Crosby
FlyBase
Subject: rhl gal4
Lynn,
The rhl-gal4 is an pGawB insertion that was mapped by in situ to polytenes
to the region where rhomboid lies. Based upon this, and the fact that it
is expressed in the same pattern as rho, it was given the name
'rhomboid-like gal4' or 'rhl-gal4'. It is a viable insertion, and so it
has likely not landed in the rhomboid gene itself, so we couched our naming
of it to reflect that it's near rho, and is expressed like rho.
I hope this info. is what you need.
BN
Elizabeth Noll
Harvard Medical School-HHMI
Department of Genetics
Perrimon Lab
200 Longwood Ave.
Boston, MA 02115