Subject: Helping FlyBase
Hi Steve,
how are you? Well I hope.
I'm writing about your latest Genetics paper:
Martin-Morris et al, Genetics 147: 671--677
Heterochromatic trans-inactivation of white ....
on page 674, column 1, under 'mini-white transgenes can be inactivated' it says
'These (three) transposon insertions are alleles of the Lighten-up gene
(A.K. Csink, unpublished results).'
I would like to know the allele symbols for these Lip mutations, so that we
can record the P{lacW} insertions with symbols that will match their future
publications, and so avoid duplicate entries in FlyBase.
Many thanks for your help,
with best wishes,
Rachel.
>
Subject: Re: Helping FlyBase
Rachel,
I'm responding (late, I apologized) to your e-mail to Steve Henikoff
concerning the Lighten-up alleles described in our recent Genetics paper
(October). I'm not certain how to designate the alleles. I received the
insertions from the Berkeley Genome project and defined them as Lip alleles
because they are 1) in the correct region 2) are lethal over other Lip
alleles I've isolated and 3) dominantly enhance the wbl mutation. Is
there any preferred standard for naming alleles from insertions collected
by the the genome project? If not I would call them Lip3D2, Lip3D5 and
Lip14E8.
Sincerely,
Amy Csink
Fred Hutchinson Cancer Research Center
1100 Fairview Avenue N, A1-162
P.O. Box 19024
Seattle, WA 98109-1024 USA
>
Subject: Re: Helping FlyBase
Hi Amy,
Thanks for your mail.
>I received the
>insertions from the Berkeley Genome project
ah ha. Very interesting. I guess that the three alleles you have
correspond to these three alleles of the gene FlyBase currently has as
'l(3)01086':
l(3)01086j3D2
l(3)01086j3D5
l(3)01086j14E8
We prefer to keep the genome project allele designations, so we will rename
this gene as Lip, using your message as the personal communication that
indicated the identity of l(3)01086 with Lip, with the alleles being
Lipj3D2
Lipj3D5
Lipj14E8
How's that? These are very close to your preferred symbols, and in line
with the way we generally treat genome project alleles.
Thankyou for the information. Its great when we can merge the rather
anonymous genome project alleles with phenotypically exciting ones!
with best wishes,
Rachel.