Subject: ADRC: 368C
Hi Matt,
I am writing in connection with your
abstract for the upcoming Washington DC ADRC:
'Genes controlling cytoskeletal behavior in syncytial embryos.'
You describe two genes that are new to FlyBase, quag and lag. Do you have
cytological locations for them? It is nice if we can keep as
many gene records as possible anchored to the map.
Thank you for your help,
with best wishes,
Rachel.
Subject: Re: ADRC: 368C
Hi Rachel-
The gene name 'laggard' was my original name for 'fray'. It is required
required maternally for normal mitoses in syncytial embryos.
The gene I'm calling 'quagmire' maps to 94A8-B5 by a combination of
deficiency mapping and meiotic mapping between P elements. It was reported
previously as l(3)05089, which is one of the chromosomes from the Spradling
collection of P lethals, and which is described as having a maternal effect
in germline clonal analysis in Perrimon et al. (1996) Genetics. The P
element maps to 91A, but itself is not associated with a lethal, even
though it is inserted in the leader sequence of a known gene. The only
lethal on that chromosome is the one I've mapped to 94A-B. It is very
likely to be a mutation in the Drosophila homolog of mouse Tcp-1, although
I haven't yet proven this.
By the way, if quagmire and Tcp-1 turn out to be the same, do I have to go
with the name Tcp-1? Mine would be the first mutation identified in the
locus. It was only identified previously as a cDNA homologous to the mouse
gene.
That's about it.
Best regards,
Matt