Zhiqiang Han
Re: ADRC: 014
Dear Zhiqiang,
I am writing in connection with your abstract for the upcoming Washington
DC ADRC:
'A conserved p38 MAP kinase pathway regulates Drosophila immunity gene expression'
You describe Mkk3 and Mkk4, both new to FlyBase
Abstract 344C (Takaesu et al, 'A novel p38 MAPK cascade,
DmMEKK1-DmMKK3-DmMPK2, may function in Dpp/TGF-beta signaling pathway in
Drosophila') also describe an 'MKK3'. Do you know whether this is the same
gene as your Mkk3?
Also, FlyBase has this gene entry already:
\*a Mek3
\*x FBrf0085669 == Noselli and Glise, 1996, A. Conf. Dros. Res. 37: 354
\*x FBrf0086756 == Noselli, 1996.4.28, personal communication
\*x FBrf0100395 == Suzanne, 1997, Int. J. Dev. Biol. 41(5): 28S
\*z FBgn0015763
\*c 11D1--11D2
\*c Left limit from (method unavailable) (FBrf0086756)
\*c Right limit from (method unavailable) (FBrf0086756)
\*c 11D1--11D2
\*e MEK-kinase 3
\*i DMEK3
\*j species == Mus; gene == Prkmk1; MGI:102562
\*j species == Homo sapiens; gene == PRKMK1;  GDB:136418 ;  OMIM:176872 
\*F protein kinase == EC 2.7.1.37
\*d MAP kinase kinase (MAPKK)
\*i DMEK3
Does this correspond to your Mkk3?
Do you have a cytological location for Mkk3 or Mkk4? It is nice if we can
keep as many gene records as possible anchored to the map.
Thank you for your help,
with best wishes,
Rachel.
Subject: gene: MKK3 and MKK4
Dear Rachel:
I went to fly meeting but didn't get chance to talk to other
people to know if the MKK3 that I have is the actually same as other
people's. The D-MKK3 I have is about 1 kb away
from hep, 5' to 5'. It is in the region 11C4-D3 on X chromosome. D-MKK4
is in the region of 85A2-A7 on 3rd chromosome. I checked Drosophila gene
bank but didn't find any sequences for MKK3 except for some sequence tags. I
would assume that my
D-MKK3 is the same as the one Noselli and Takaesu have based upon they
are all mostly homologous to human MKK3 and MKK (65%). But I don't have
sequence alignment to confirm it.
Best regards
Zhiqiang Han
Subject: Re: gene: MKK3 and MKK4
Dear Zhiqiang,
In view of the nature of the encoded product and cytological location I
will treat your D-MKK3 as the same gene as Mek3 in FlyBase.
I will curate your mail as a personal communication to FlyBase to serve as
a source for the lovely mapping data. I am presuming that you used
chromosome in situ hybridization to determine the cytological locations.
Please let me know if this is not the case.
With best wishes,
Rachel.