Subject: Gal4 line 109-68
We wish to add to the flybase some details about an enhancer trap Gal4
line 109-69 (FlyBase ID number: FBal0052395, transposon flybase ID
number: FBti0004036). This line was isolated in Jan's lab, and was used in several
publications. The most precise description of this line is by Dan
Doherty's 1997 paper (Doherty, D., Jan, L.Y., Jan, Y.N. Development v124,
3881--3893. Flybase ID FBrf0099033), i.e. 'it is expressed in a subset of
proneural cluster cells, SOP's and their daughters '. In our hands, 109-68
is expressed in some proneural cluster such as the ones in the scutellar
region and predominantly (if not exclusively) in the SOP and their
daughters for most of the bristles elsewhere in the notum. In making
mosaics with UAS-flp, and 109-68, we can get clones that are only in the
sense organ lineage (the surrounding epidermal cells were marked so we
know). There are also cases in which the clones include nearby epidermal
cells. In other publications, we have stated that 109-68 is SOP specific,
which appears to be a over-simplification. We hope that by providing this
information through the flybase, the potential confusion on this line can
be minimized.
Thank you.
Chaoyang Zeng Ph. D.
Jan's Lab
University of California at San Francisco