Personal communication from: Akira Chiba
To: Bloomington Drosophila Stock Center
Subject: P{UAS-gapGFP}
Information communicated:
"My lab has generated P(UAS-gapGFP) transformant flies. gapGFP is a modified GFP, a fusion of the myristylatioin sequence from GAP43 gene to GFP, that is designed to target the GFP to cell memebrane. At the recent CSH drosophila neurobiology meeting, we reported their use in visualizing the fine memebrane structures of various cell types such as neurons and muscles."
Additional information provided by A. Chiba:
The construct is a modification of P{GawB}, starting with pwG17 from the C. Goodman lab. The myristylation sequence is from the human GAP43 (as described and constructed by Moriyoshi et al. NEURON (1994) 16:255-260 ). The GFP itself has the S65A mutation (per Morisyoshi et al.) and is visible with a regular FITC filter set.