FlyBase Recombinant Construct Report: P{UAS-gapGFP}

General Information

Symbol

P{UAS-gapGFP}

FlyBase ID

FBtp0001558

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^{UAS.Tag:Palm(hGAP43)}

Also Known As

UAS-GAPGFP, UASGAPGFP, UAS-Gap-GFP, UAS GAP-GFP, UAS-GAP43-GFP, UAS GAP GFP, UAS-myristylated-GFP

Size

Expression Data

Associated insertion(s)

4 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Avic\GFP^{UAS.Tag:Palm(hGAP43)}

Transgenic product class

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

GFP(S65T)

green fluorescent protein

Tagged with

Tag:Palm(hGAP43)

plasma membrane protein localization tag

Description

UAS regulatory sequences drive expression of the GFP(S65T) fluorescent protein tagged with a membrane targeting signal (Tag:Palm(hGAP43)).

(Chiba, 1998.5.20)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{UAS.Tag:Palm(hGAP43)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

report comment: GFP is the more active S65A variant.

(Chiba, 1998.5.20)

construct_comment: Designed to target GFP expression to the cell membrane. Carries the myristylation sequence tag T:Myr2 and the S65A variant of GFP.

(Chiba, 1998.5.20)

Stocks (3)

Stocks containing insertion(s) of this recombinant construct

Bloomington

4522

w^*; P{UAS-gapGFP}AC1

92179

w^*; sns^s660 P{UAS-gapGFP}10

Kyoto

107776

w^*; P{UAS-gapGFP}AC1

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

GAP-GFP

(Guha et al., 2009)

P(UAS-gap^GFP)

(Ritzenthaler et al., 2000)

P{UAS-GFP.Myr2}

(FlyBase, 1996-)

P{UAS-GFP.gap}

(FlyBase, 1996-)

P{UAS-gapGFP}

(FlyBase, 1996-)

UAS GAP GFP

(Hidalgo et al., 2001, Booth et al., 2000)

UAS GAP-GFP

(Guha et al., 2009, Guha and Kornberg, 2005)

UAS GAPGFP

(Griffiths and Hidalgo, 2004)

UAS myristoylated GFP

(Greco et al., 2001)

UAS-GAP-GFP

(Figeac et al., 2010, Hiramoto et al., 2000)

UAS-GAP43-GFP

(Lee et al., 2003)

UAS-GAP::GFP

(Ang et al., 2003)

UAS-GAP:GFP

(Daniels et al., 2008)

UAS-GAPGFP

(Broihier et al., 2004, Broihier and Skeath, 2002, Jarecki et al., 1999)

UAS-GFP-Gap43

(Jiang et al., 2007)

UAS-GFP.GAP

(Brown and Castelli-Gair Hombria, 2000)

UAS-Gap-GFP

(Haralalka et al., 2011, Roegiers et al., 2001)

UAS-Gap43-GFP

(Buch et al., 2008)

UAS-gapGFP

(Miller and Carlson, 2010, Tokusumi et al., 2010, Zhuang et al., 2009, Kim et al., 2003, Kim et al., 2002, Sato and Kornberg, 2002)

UAS-mGFP

(Kohsaka et al., 2007)

UAS-myristylated-GFP

(Inaki et al., 2010, Kohsaka and Nose, 2009)

UASGAP-GFP

(McIlroy et al., 2013)

UASGAPGFP

(Kinrade and Hidalgo, 2004)

UASgapGFP

(Siechen et al., 2009, Schulte et al., 2003)

mGFP

(Kohsaka et al., 2007)

pUAST-GAP43-GFP

(Lee and Kolodziej, 2002)

Secondary FlyBase IDs

References (38)

Report Sections

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