Subject: FlyBase query
Dear Dr. Haenlin,
I am curating a paper for FlyBase (Baker and Yu, 1998, Mech. Dev. 74(1,2):
3--14) in which a Dl-enhancer trap construct isolated by you and J.A.
Campos-Ortega was used.
FlyBase has a record of a number of Dl-enhancer trap constructs but I don't
think that that these correspond to your construct.  I wrote to the
corresponding author on the paper (N. Baker) and he suggested I write to
you about the details of the construct.
I would be grateful if you could tell me a few details of the construct so
that I can confirm whether this construct is new to FlyBase.  If it is new
to FlyBase I would like to include the details in FlyBase as a personal
communication from you, if possible.
1. is there a reference where this construct has been published.
2. is there a designation/line number for the construct e.g. A101, 64B
3. which enhancer trap vector, e.g. P{PZ}, P{lacW} is used
I look forward to hearing from you,
Gillian Millburn
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
>
>Dear Dr. Haenlin,
>
>I am curating a paper for FlyBase (Baker and Yu, 1998, Mech. Dev. 74(1,2):
>3--14) in which a Dl-enhancer trap construct isolated by you and J.A.
>Campos-Ortega was used.
>
>FlyBase has a record of a number of Dl-enhancer trap constructs but I don't
>think that that these correspond to your construct.  I wrote to the
>corresponding author on the paper (N. Baker) and he suggested I write to
>you about the details of the construct.
>
>I would be grateful if you could tell me a few details of the construct so
>that I can confirm whether this construct is new to FlyBase.  If it is new
>to FlyBase I would like to include the details in FlyBase as a personal
>communication from you, if possible.
>
>1. is there a reference where this construct has been published.
>2. is there a designation/line number for the construct e.g. A101, 64B
>3. which enhancer trap vector, e.g. P{PZ}, P{lacW} is used
>
Dear Gillian
I sendet two lines; both of them are insertion of  P{lacW}  in the promotor
region of Delta at about the same site (less as 1 kb).
For the 8c3 (originating from the Jan's Lab as I know) the LacZ
transcription orientation is the same as Delta (the Dl revF10 is a
revertant of this enhancer trap line), and for the 1282 (from Seugnet and
Haenlin) is in the opposite direction.
It exist as I know no references for these lines.
If you have further questions, no problem
regards
Haenlin Marc
Centre de Biologie du Developpement
Batiment 4R3
118, route de Narbonne
31062 Toulouse
>
Subject: Re: FlyBase query
Dear Marc,
thankyou for your reply, it was very helpful.
I think I have found the 8c3 line in FlyBase - we have a P{lacW} insertion
in Dl called j8C3 which comes from the Berkeley Genome Project - and I
think the j means that it came from the Jan's lab.
the 1282 line is new.
I would like to include the details from your e-mail about these lines in
FlyBase as a personal communication to FlyBase from you.
Would that be OK ?
Gillian
>
Subject: Re: FlyBase query
>Dear Marc,
>
>thankyou for your reply, it was very helpful.
>
>I think I have found the 8c3 line in FlyBase - we have a P{lacW} insertion
>in Dl called j8C3 which comes from the Berkeley Genome Project - and I
>think the j means that it came from the Jan's lab.
>
>the 1282 line is new.
>
>I would like to include the details from your e-mail about these lines in
>FlyBase as a personal communication to FlyBase from you.
>
>Would that be OK ?
>
Dear Gillian
no problem