Dear Christian,
We have just received the GenBank sequences AJ006207 and AJ006208,
submitted by your group, which are described as flanking A2(2nd)27.
We do not have a record of that insertion causing a mutation in
any gene, but I see that the unpublished reference included in the
Genbank records mentions blw in the title. It would be very helpful
for our data integrity if you could tell me whether A2(2nd)27 is in
fact an allele of blw (or of any other gene).
Many thanks in advance,
Aubrey de Grey
FlyBase
>
Subject: A2(2nd)27
Dear Dr. de Grey,
I am a PhD-student in Christian Lehners Lab. Christian asked me to answer
your mail about the A2(2nd)27 P-element insertion. This insertion indeed is
an allele of blw, according to complementation tests that we have performed
using lethal EMS-alleles (from a screen we have done in the 59AB-region)
and various P-element lines including the original blw-allele blw(1).
A(2)2nd27 and blw(1) are male sterile over themselves, over Df(2R)59AB and
over two out of eleven lethal EMS-alleles comprising one of four
complementation groups from our screen. (We just tested only two of the
eleven alleles for complementation with the P-insertions). According to
sequence comparisons, A(2)2nd27 seems to reside in the 5'-UTR of the gene
encoding the alpha subunit of the mitochondrial ATP-synthase of Drosophila.
(The reference included in the Genbank-records is in press meanwhile and
will soon be published in 'Molecular and General Genetics').
I hope this information will be of some help,
best regards,
Henning Jacobs
Department of Genetics
Lehner-Lab
University of Bayreuth
>
Subject: Re: A2(2nd)27
Dear Henning,
thankyou for the information about the A2(2nd)27 insertion. Aubrey has
given me your e-mail to curate as a personal communication to FlyBase from
you - is that OK ?
Also, at the end of your e-mail you say:
'A(2)2nd27 seems to reside in the 5'-UTR of the gene encoding the alpha
subunit of the mitochondrial ATP-synthase of Drosophila'
FlyBase has a record of a gene with the symbol ATPsyn-alpha, which is
stated to encode the mitochondrial ATP synthetase alpha chain. The
information we have about this gene is:
Accession numbers: AA801788, Y07894, Q94512, P35381.
References:
Santaren et al., 1993, Exp. Cell Res. 206(2): 220--226
Talamillo et al., 1998, Molec. Biol. Rep. 25(2): 87--94
Do you know whether this ATPsyn-alpha is the same as the one encoded by
blw? If it is, I can merge the 2 genes, keeping blw as the valid symbol,
I look forward to hearing from you,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
> 
> Subject: A2(2nd)27
>
> Dear Dr. de Grey,
>
> I am a PhD-student in Christian Lehners Lab. Christian asked me to answer
> your mail about the A2(2nd)27 P-element insertion. This insertion indeed is
> an allele of blw, according to complementation tests that we have performed
> using lethal EMS-alleles (from a screen we have done in the 59AB-region)
> and various P-element lines including the original blw-allele blw(1).
> A(2)2nd27 and blw(1) are male sterile over themselves, over Df(2R)59AB and
> over two out of eleven lethal EMS-alleles comprising one of four
> complementation groups from our screen. (We just tested only two of the
> eleven alleles for complementation with the P-insertions). According to
> sequence comparisons, A(2)2nd27 seems to reside in the 5'-UTR of the gene
> encoding the alpha subunit of the mitochondrial ATP-synthase of Drosophila.
> (The reference included in the Genbank-records is in press meanwhile and
> will soon be published in 'Molecular and General Genetics').
>
> I hope this information will be of some help,
> best regards,
>
> Henning Jacobs
>
> Henning Jacobs
> Department of Genetics
> Lehner-Lab
> University of Bayreuth
> ----- End Included Message -----
>
> Subject: Re: A2(2nd)27
>Dear Henning,
>
>thankyou for the information about the A2(2nd)27 insertion.  Aubrey has
>given me your e-mail to curate as a personal communication to FlyBase from
>you - is that OK ?
>
>Also, at the end of your e-mail you say:
>
>'A(2)2nd27 seems to reside in the 5'-UTR of the gene encoding the alpha
>subunit of the mitochondrial ATP-synthase of Drosophila'
>
>FlyBase has a record of a gene with the symbol ATPsyn-alpha, which is
>stated to encode the mitochondrial ATP synthetase alpha chain.  The
>information we have about this gene is:
>
>Accession numbers: AA801788, Y07894, Q94512, P35381.
>
>References:
>
>Santaren et al., 1993, Exp. Cell Res. 206(2): 220--226
>Talamillo et al., 1998, Molec. Biol. Rep. 25(2): 87--94
>
>Do you know whether this ATPsyn-alpha is the same as the one encoded by
>blw? If it is, I can merge the 2 genes, keeping blw as the valid symbol,
>
>I look forward to hearing from you,
>
>Gillian
>
Dear Gillian,
\- To curate the information as a personal communication to FlyBase is o.k.
\- The ATPsyn-alpha gene (accession number Y07894) described in Talamillo et
al.,        1998 indeed is the same gene.
I hope this information is of some help.
Henning