Hi Martin, I am curating an abstract of yours, from the Sixth European meeting on the Neurogenetics of Drosophila, for FlyBase: Albert et al, 1997, J. Neurogenetics 11: 141. I have a few questions I would like to ask you, your answers will clarify the data for loading into FlyBase. 1) In this abstract you discuss chromosome Df(2L)R70. Is this the deficiency segregant of Tp(2;Y)R70, the deficient region being between 21C3;21C5? If not, could you please describe the chromosome. 2) You describe a P-element insertion, P21BC. Is this a natural P-element or an engineered construct? Do you have any further information regarding insertion point? 3) Has the mbm mutation in line cn bw sp been previously described or is this new information? Have you written a paper since this abstract, maybe that will answer these questions? Many thanks for any help you can provide. On the advice of Stephan Schneuwly I have emailed you to help as I have neither a fax number or email address for S. Albert or T. Twardzik. Regards, Eleanor Whitfield FlyBase > Subject: Re: Help FlyBase - mbm abstract Dear Eleanor: Thanks for your inquiry. I will go through your questions one by one. 1) Yes. This is the deficiency. We received it from Pasqual Heitzler, Stassbourg. 2)The P-element( PlacW 319/11) is from a screen conducted in collaboration with Ernst Hafen in Zurich. It contains a lacZ and a mini-white gene. It is inserted just to the right of a Hin dIII site in a 1.3kb H/E fragment in the genomic region of the phage BPal 14. This fragment contains an alternatively spliced exon of the plc21 gene. The insertion site is just to the left of the exon. All this is in a Doctoral Thesis of Susanne Albert which, however, is in German. 3) This is new and yet unpublished information. Part of the masses of data is in a paper by Albert et al. (1997) Isolation and characterization of the droPIK57 gene encoding a new regulatory subunit of phosphatidylinositol 3-kinase from D.m.. GENE 198, 181-189. (See also: Weinkove et al. (1997) p60 is an adaptor for the D. phosphoinositide 3-kinase, Dp110. J.Biol. Chem. 272, 14606-10). If you need any further information, do not hesitate to contact me again. All the best, Martin Heisenberg \------------ Prof. Dr. Martin Heisenberg Theodor-Boveri-Institut fuer Biowissenschaften Lehrstuhl fuer Genetik Am Hubland 97074 W u e r z b u r g
