Dear Dr. Pignoni,
I am curating a paper for FlyBase:
Huang and Kunes, 1998, Development 125(19): 3753--3764
in which they used a 'flp-out' GAL4 line that was referenced to your paper:
Pignoni and Zipursky, 1997, Development 124(2): 271--278
I wrote to Larry Zipursky with some questions about the line and he
suggested I write to you as you made the construct.
the line Huang and Kunes used in the paper was called 'PTub>CD2>GAL4'.
FlyBase does not have a record of this construct - we have a similar
construct where the promoter is Actin 5C - PActin>CD2>GAL4 (from your
1997 Development paper), but not tubulin.
I would be grateful if you could tell me some details about the
construct, so that I can include the details in a personal
communication from you to FlyBase.
1. which particular tubulin promoter is used (i.e alpha1, beta2 etc.)
2. is the FRT cassette containing CD2 the same as used in the
PActin>CD2>GAL4 construct from Pignoni and Zipursky, 1997,
Development 124(2): 271--278 ?
3. what vector is the construct in, e.g. CaSpeR, Carnegie20 (so I can
work out which marker gene is in the construct) ?
4. who made the construct (this helps in naming the construct in
FlyBase) - I guess this was you?
I look forward to hearing from you,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
>
Subject: RE: FlyBase query
Dear Gillian
> 1. which particular tubulin promoter is used (i.e alpha1, beta2 etc.)
	Tubulin alpha 1
> 2. is the FRT cassette containing CD2 the same as used in the
> PActin>CD2>GAL4 construct from Pignoni and Zipursky, 1997,
> Development 124(2): 271--278 ?
	yes, identical
>
> 3. what vector is the construct in, e.g. CaSpeR, Carnegie20 (so I can
> work out which marker gene is in the construct) ?
	CaSpeR 2 , mini-white
>
> 4. who made the construct (this helps in naming the construct in
> FlyBase) - I guess this was you?
	Yes, I did
	Francesca