Subject: Df(2L)S2590 info
Hi Rachel--
Here is a little information about Df(2L)S2590 that Todd said I could
forward to you. We will be incorporating this Df strain into the B'ton
collection soon.
Best regards,
Kevin
>Hi Kevin,
>Yes, we have l(3)L2249 and will send both you and Jeff a copy.
>
>On another note, I have some more info on Df(2L)S2590 that you were talking
>to Amy about recently. I'm not sure what she has told you but I'll tell
>you what I know and what I recently found out. First, it is not a Scott
>line, it came from a Ras screen in the lab and was X-ray induced. The
>cytology of the breakpoints 23D2; 23E3 should be pretty accurate since the
>deficiency junction was cloned from the S2590 stock, labeled and hybridized
>back to wild type chromosomes. The result was two signals, one at 23D1-2
>and the other 23E3-4. This was done with two independent clones.
>Now this is what I learned recently and where it gets confusing for me and
>clouds up the picture a bit. The deficiency junction was sequenced and it
>breaks in the toucan gene on the 23D1-2 end, were the last ~4300 bp is
>missing in S2590 stock. The confusing part is the gene mad, which is
>distal to toucan, is also missing in S2590.
>
>l(2)k00237, which is a P allele of mad, fails to complement Df(2L)S2590 and
>a southern with S2590 genomic DNA with a mad cDNA probe, shows mad is
>missing in S2590. This implies that there is a small (not cytologically
>visible) second aberration on the D(2L)S2590 chromosome. All this work was
>done by Allan Wong, an undergraduate in the lab, and Marc Therrien, a
>postdoc in the lab. They have stop working on this project since what
>appeared at first to be a simple side project had grown in to a more
>complicated project they chose not to pursue.
>
>As always, if you have any question, let me know and I try and find the
answers.
>Take care,
>Todd
>
>Todd Laverty
>Rubin Lab