Subject: Re: Gli
rachel,
a little extra data on Gli alleles. This email from Vanessa Auld says that
AE2 and J29 are viable alleles (not stated in FB).
Also the excisions of AE2 - GliAE2Δ45 and
GliAE2Δ4b - are lethal alleles derived from AE2;
and GliAE2Δ4a is a viable allele.
J
>
>Subject: Re: Gli
>
>Dear John,
>The P induced Gli alleles were not lethal insertions. They are totally
>viable because they are inserted in the first intron of the gene. The P
>elements are the P<up>lacZ, w+</up> element for AE2, J29 and the P<up>lacZ, ry+</up>
>element for rL82. I made a series of deletion alleles of gliotactin using
>the Pelement insertion and these deletions were lethal and protein nulls
>(called delta45, delta4b). As well at the same time I made a precise
>excision using the same P element insertion and this allele is viable and
>called delta4a.
>
>Hope this is helpful,
>Vanessa.
>
>====================================================================
>Dept. Zoology
>6270 University Blvd.
>University of British Columbia
>Vancouver, B.C.
>Canada V6T 1Z4
>====================================================================
>