Subject: FlyBase query
Dear Dr. Jongens,
I am curating your paper for FlyBase:
Faulkner et al., 1998, Dev. Genet. 23(4): 264--274
I have a question about the P{lacW} insertion - pngcl - that you used
to make excisions removing parts of the cmp44E gene.
I would be grateful if you could tell me the identifier number of this
line which you obtained from Gerald Rubin's lab, e.g. k02210, k03408
etc. as we probably already have a record of this line in FlyBase and I
would like to be able to record the following information under the
correct P{lacW} line.
1. This information will be useful in recording that this P-element
insertion was the progenitor for the cmp44E alleles you created.
2. In addition, although this insertion does not cause a phenotype, I
will create an allele of cmp44E for the insertion because it is
inserted in the transcription unit (I will also record the information
that the line does not cause a phenotype).
3. Also as this line originally had 2 P-element insertions, this will
also be useful information to have for the line.
I look forward to hearing from you,
Gillian
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Gillian Millburn.
FlyBase (Cambridge),
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Subject: Re: FlyBase query
Dear Gillian,
	The line from which we obtained pngcl was k00114. We recombined
away the lethality and the other P-element from the P-element insertion at
44E3,4(pngcl). If you have further questions don't hesitate to ask.
							Tom Jongens
Thomas A. Jongens
Dept. of Genetics
University of Pennsylvania
School of Medicine
709b Stellar-Chance Labs
422 Curie Blvd
Philadelphia, Pa 19104-6100