Trunova, S.A., Dubatolova, T.D., Omel'ianchuk, L.V. (1998). [Determination of the expression phase of chb(V40) gene in the cell cycle of Drosophila melanogaster.]  Ontogenez 29(5): 342--346.

FBrf0107386

Research paper

Using autoradiography, we have determined cell cycle parameters in neuroblasts of III-rd instar larvae of D. melanogaster. The overall duration of the cell cycle is 9 h, tG1 = 4 h, tS = 3.5 h, tG2 = 1 h, and tM = = 0.5 h. Using histochemical staining for beta-galactosidase activity, we have determined the stage of expression of the reporter gene of P[1ArB] element inserted into chb gene. The chb gene, which codes for a centrosomal protein, is expressed during the last third of S phase. This result illustrates the feasibility of using the enhancer trap P[1ArB] to study differential expression of cell cycle genes.