Subject: ADRC-682C
Dear Terence,
We are currently curating the abstracts for the upcoming Bellevue
(Seattle) Annual Drosophila Research Conference, for FlyBase. I am
writing in connection with your abstract:
'Analysis of the roles of a family of Drosophila skp1 homologs in cell
cycle progression'
You mention four genes that are new to FlyBase, skpA, B, C and D.
Do you have a map location for any of these genes? It is nice if we
can keep as many gene records as possible anchored to the map.
Thank you for your help,
with best wishes,
Rachel.
Subject: Re: ADRC-682C
Dear Rachel,
Thanks for your interest in the Drosophila skp genes. A lot of the
information for dskpA ties into the Berkeley database. EP(X)1423 is
inserted into the 5' UTR of dskpA, originally indicated by end sequencing
done by the BDGP. The sequence from EP(X)1423 also ties into the sequence
of cosmid 115C2, in which the EDGP identified an ORF homologous to SKP1.
There are also a bunch of ESTs, but I'm not sure you keep those on the
Flybase map. I'll add that dskpA is located at 1B10-14 on the polytene
map, so you can also anchor that cosmid sequence. Imprecise excisions of
EP(X)1423 are lethal, and fail to complement:
Df(1)svr
T(1;Y)B106, y (the Df(1) half)
Df(1)su(s)83
but are complemented by:
Df(1)y74k24.1
The complementation data is consistent with the polytene localization.
As far as the other homologs, I'll send along that information when I
publish them, since I know other people are interested in the SKP1 genes
and may not know about the other family members. I have no objections to
you adding the above data to Flybase at this point, since almost all of it
can be derived from the BDGP data anyway (except for the polytene
localization). Let me know if you want any other pieces of data clarified.
\-Terence
Subject: Re: ADRC-682C
Dear Terence,
Thank you very much for the information about skpA. It is very useful
indeed and allows me to rename the  EG:115C2.4  gene (FBgn0025637) that
is currently in FlyBase as skpA. I see no reason to doubt that they
are the same thing. Not only that, but I can link the insertion we
currently have as P{EP}EP1423 (FBti0007441) to an allele of skpA, which
we should call skpAEP1423, to reflect the insertion into the 5' UTR
(even if the insertion is phenotypically silent).
Brilliant.
We do indeed capture links between ESTs and genes so if it is easy for
you to provide me with the relevant EST identifiers that would be the
icing on the cake.
Your mail will be curated as a personal communication to FlyBase.
Many thanks,
Rachel.