Subject: ADRC-572A Dear Yong, Hi there and how are you doing? We are currently curating the abstracts for the upcoming Bellevue (Seattle) Annual Drosophila Research Conference, for FlyBase. I am writing in connection with your abstract: 'The Drosophila eye color mutant ruby encodes 3 subunit of AP3 complex and is involved in synaptic transmission.' You say that the rb subunit of 'AP3' was identified from an EST. Please can you tell me which EST, as these are the kinds of links we need to make? Thank you for your help, with best wishes, Rachel. Subject: Re: ADRC-572A Dear Rachel These two overlapping ESTs are LD08782 and LD22852, and the EMBL entry for beta3 subunit of AP3 is AJ011778, plus a related sequence AJ131729, which is the distal breakpoint of In(1)rb-D1. The distal breakpoint of In(1)rb-D1 disrupts the coding region of beta3 subunit. With best wishes, Yong