Subject: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101
Rachel,
Another note for FlyBase:
From the cytology one would predict that
Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 was a deletion from the GT6 breakpoint
to TE35B i.e. Df(2L)34F3;35B1.2. Genetically it should be deleted for wb,
ms(2)34Fe, l(2)34Fc, l(2)34Fd, l(2)35Aa, el, pu and noc.
In fact it is hypomorphic for wb, l(2)34Fc, l(2)34Fd, l(2)35Aa and
wild-type for pu.
Over el1 it is phenotypically el.
Over ms(2)34Fe1715 it is male fertile - but hard to score degrees of male
fertileness.
I got a similar result from the strange segregant
Ts(2Lt;4Lt)GT6+Ts(2Rt;Y)TE35B-18.
GT6 itself is hypomorphic for wb.
Therefore I assume that GT6 is a complex event which carries a
heterochromatic duplication of wb-pu.
John
Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101
Thanks John,
I'll curate your message as a pc from you to us - The fact that you
get consistent results in your complementation tests suggests that the
heterochromatic duplication is into the bit of the 4th that is included
in Ts(2Lt;4Lt)GT6, doesn't it?
Has anybody mapped the breaks molecularly?
Rach
Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101
> The fact that you get consistent results in your
>complementation tests suggests that the heterochromatic duplication is
>into the bit of the 4th that is included in Ts(2Lt;4Lt)GT6, doesn't it?
Yes, indeed it does - well spotted.
>Has anybody mapped the breaks molecularly?
Yes. Terry D. will send me his best guess for this.
Rootles
Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101
Hi John,
me again.
Have you, by any chance, complementation tested T(2;4)GT6 itself for
hypomorphic-ness at l(2)34Fc, l(2)34Fd, l(2)35Aa?
Rach
Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101
Hiya,
me again.
>Have you, by any chance, complementation tested T(2;4)GT6 itself for
>hypomorphic-ness at l(2)34Fc, l(2)34Fd, l(2)35Aa?
I have - T(2;4)GT6 is hypomorphic for wb and nothing else.
I think it must involve extra breaks. T(2;4)GT6 is broken (cytologically,
molecularly and genetically) somewhere near wb.
This putative variegating Dp only becomes 'noticeable' in the absence of
Ts(2Rt;4Rt)GT6 (or should I say in the absence of 34Fc, 34Fd, etc.).
J