Subject: FlyBase query
Dear Sarah,
I am curating your paper for FlyBase:
Jennings et al., 1999, Molec. Cell. Biol. 19(7): 4600--4610
In this you use a 'sal-Gal4' line which you reference to 2 papers:
de Celis et al., 1997, Development 124(10): 1919--1928
Thomas et al., 1995, Genetics 139(1): 203--213
We do not have a record for a sal-Gal4 line in either of these papers,
but both papers use the '459.2' Gal4 line.
Is the 459.2 line actually an enhancer trap in a spalt gene which you
have used in the Molec. Cell Biol. paper ? If so, do you know whether
the insertion causes a mutation in spalt or is it just reflecting the
expression of the spalt gene without causing a mutant phenotype ?
Also, which spalt gene is it inserted in - spalt major, spalt adjacent
or spalt related ?
If the sal-Gal4 line you used in the Molec. Cell. Biol. paper is not
459.2, I would be grateful if you could tell me some details about the
line. Is it an enhancer trap line or a promoter fusion in which a
spalt promoter drives Gal4. If it is an enhancer trap, is it a P{GawB}
insertion ? If it is a promoter fusion, which vector is it in e.g.
CaSpeR, Carnegie 20 (this helps to work out the marker gene - w or ry
in the construct). Again which spalt gene is it - spalt major, spalt
adjacent or spalt related ?
I look forward to hearing from you,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
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>
Subject: Re: FlyBase query
Dear Gillian,
Re your query:
>
>Jennings et al., 1999, Molec. Cell. Biol. 19(7): 4600--4610
>
>In this you use a 'sal-Gal4' line which you reference to 2 papers:
>
>de Celis et al., 1997, Development 124(10): 1919--1928
>Thomas et al., 1995, Genetics 139(1): 203--213
>
>We do not have a record for a sal-Gal4 line in either of these papers,
>but both papers use the '459.2' Gal4 line.
>
>Is the 459.2 line actually an enhancer trap in a spalt gene which you
>have used in the Molec. Cell Biol. paper ?
Yes.
Insertion is homozygous viable: no phenotype observed.
>Also, which spalt gene is it inserted in - spalt major, spalt adjacent
>or spalt related ?
No idea I'm afraid. Pattern looks like spalt major as far as I have seen,
but we haven't really examined it in sufficient detail to be able to
distinguish. As far as I know it isn't mapped moleclarly.
bye for now,
Sarah.
Sarah Bray
Dept. of Anatomy,
Univ. of Cambridge,
>
Dear Sarah,
thanks thats great ! I'll make the information that 459.2 is a sal-Gal4
line a personal communication from you to FlyBase.
I'll put it under spalt major for now,
Gillian