Subject: FlyBase Query (W88)
Dear Dr Heitzler,
I am curating a paper for FlyBase:
Franc et al. Science, 1999 284(5422) 1991--1994.
I have been correspondence with Nathalie Franc about this paper. In
response to the question below, she suggested I get in touch with
you, since you had generated the W88 line.
1.Df(2L)TE99(Z)XW88
In your paper you use a Deficiency which you call W88. We have no
record of this aberration and you did not describe its generation.
Could you tell me where this aberration came from?
If this line has been used in another paper, it would be useful to know
which one and what name was used. If not, then any genetic information
about this line would be good, mutagen, breakpoints etc.
Any information that you give will be entered into FlyBase as a
personal communication from you.
Thank you for your help,
Chihiro
Subject: Re: FlyBase Query (W88)
Dear Dr Chihiro Yamada
Thank you very much for your message, I will try to explain this
complicated XW88 story. The TE99(Z) natural w+ transposon from Gunnar
Ising is inserted in the 21C region.
I found that this transposon is inserted within the 5' end regulatory
region of the ushaped gene and is associated with a hypomorphic viable
ush phenotype (Cubbada et al 1997
).
I performed X-ray w- derivatives from TE99(Z) (unpublished results).
Among numerous putatives, TE99(Z)XW88 was quite unusual because it
exhibits a new dominant phenotype due to ectopic ush expression
(unpublished observations). The TE99(Z)XW88 chromosome is associated
with the small deletion published in our Science paper. This chromosome is not ush- because Df(2L)TE99(Z)XW88/ush- flies show
the viable ush loss of function phenotype like for the original
TE99(Z)/TE99(Z) or TE99(Z)/ush- combinations. The best explanation for
the new dominant phenotype is that in Df(2L)TE99(Z)XW88, the ush
regulatory region juxtapose enhancer sequences of the ex gen e.
I induced revertants of the dominant phenotype of Df(2L)TE99(Z)XW88. I
found three revertants which as expected reduce further or abolish the
ushaped function; of course they retained also the original deletion. Their
characteristics are as following:
\- In(2L)TE99(Z)XW88-DV1,
X-ray induced, associated to an inversion 21C;23A, stronger viable ush
allele as the original chromosome.
\- TE99(Z)XW88-DV2,
EMS-induced, ush- chromosome
\- TE99(Z)XW88-DV3,
EMS-induced, ush- chromosome
I hope you have understand my XW88 story, please note that the deletion use
in our Science paper is in fact the chromosome that carries the EMS-induced
ushXW88-DV2 allele. Thank you for your attention.
Yours sincerely,
Pascal Heitzler.