Subject: Helping FlyBase: kst01318
Dear Graham,
You may be able to help FlyBase out. In your Development paper:
FBrf0102732 == Thomas et al., 1998, Development 125(11): 2125--2134
you mention revertants of kst01318 (P-element excisions), and say
that their eye phenotype is reverted. Is the reduced viability seen
for kst01318 restored to normal? The reason I ask is that Thuy
Nguyen told us that the 'lethality' of the chromosome is not associated
with the P{PZ}, but this may have been a misunderstanding about what
we were asking.
We need to know because it has an effect on how many mutations we
associate with the 01318 chromosome.
We'd be grateful for any help you can give us.
Regards,
Rachel.
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Subject: Re: Helping FlyBase: kst01318
Dear Rachel,
What she means is that the chromosome is homozygous lethal due to the
presence of an ancillary lethal not associated with the P-element in karst.
The karst allele associated with the P-element is actually unusually viable
in combination with most other karst alleles and Df near 63. The lethal to
which Thuy refers is somewhere in the 70's and is easily recombined away
from the kst mutation. We roughly mapped this some time back with the Df
kit I think. However, the postdoc who did this, and could tell you
precisely which Df uncovers the lethal, is away on holiday right now, but I
will copy him in on this response and he will fill you in. In the meantime
don't put anything in the database based on the above just yet....
What brought this up?
Cheers!
Graham
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Subject: Re: Helping FlyBase: kst01318
Dear Graham,
When I hear from the post doc I will curate his and your
mail as a personal communication from you and him to us, to get in the
data that kst01318 is itself viable and was originally on the same
chromosome as the l(3)... hit. If you could pass on this message to
him that would be great.
The reason that this came up was that one of the FlyBase folk (Kathy
Matthews) noticed that the kst01318 record said both 'semi-lethal'
and 'The lethality of the kst01318 chromosome is not associated
with a closely linked P{PZ} insertion.', and smelled a rat
(kst01318 is in her Stock Center).
best wishes,
Rachel.
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Subject: kst01318 and lethal locus
Dear Rachel,
With respect to the lethal associated with the kst01318 allele, I mapped
the lethal to Df (3L) 2993 which, according to Flybase, uncovers
72C01-D01;73A03-04. Hope this is what you need.
best regards
Bryce MacIver