Personal communication from:
Jeongsil Kim-Ha
Sungkyunkwan University School of Medicine
Center for Transcriptional Mediator
Seoul, Korea
Dec. 2, 1999
Query from Curator:
I have a couple of questions with respect to the data in Kim-Ha et al., 1999,
Molec. Cell. Biol. 19(4): 2505--2515.
1) Can you provide me the boundaries (restriction sites?) of the 17.1kb
genomic fragment used for rescue so I can find it in the sequence?
2) Can you give me the exact location of the P{lacW}Rbp9[k12901]
insertion so I can include it in the annotation.
3) When you say that other P elements have landed at a particular base
(e.g. P{lacW}Rbp9[P1374] inserted at nucleotide 4668), do you mean that
it inserted between 4667 and 4668 or between 4668 and 4669?
Response:
The informations you requested are as follows: 1) The 5' restriction
enzyme site is SalI and we didn't sequence this region. The seq. of 3'
site is 5'-ATGTGTGTAGTGCATAGAGAAACCG-3'/end 2) We do not know the exact
location of the P{lacW} Rbp9[k12901] as we did not sequence the
junction. We know that it is located roughly at nucleotide position
3400 by analyzing the PCR product. 3) P[2775], P[2690] and P[1374] are
inserted between 7132-7133, 7197-7198 and 4668-4669, respectively.