Subject: bdgp Pcmt localization information
Dear BDGP members,
My lab originally identified the Pcmt gene in Drosophila and we have
been doing some cross-hybridization experiments to develop a physical
map of the region (hopefully as a start for a P element hop). We now
have acquired quite a bit of information that I would like to share
with you, in the hope that it will contribute to the mapping project.
As a result of these various cross-hybridization experiments, described
below, I think we can place Pcmt confidently in 83A5-6.
We performed hybridization with cosmid contig 659. We found that both
cDNA and genomic clones for Pcmt hybridized with cosmids 80E1 and 112E7
(which appears to be contained within 80E1. Cosmid 80E1 cross-hybridizes
with cosmid 189F12, although the Pcmt gene is not included in 189F12.
We have also analyzed the position of P element insertions (using
plasmid rescue protocols with genomic DNA) on the cosmid clones. These
have shown that P1597 (also referred to as l(3)03644) is contained
within 80E1 and 189F12. The P element insertions P212 (also l(3)j5E2)
and P1664 (also l(3)05616) are present on 189F12, but not on 80E1. If
you would like a graphical depiction of the physical distances that we
have estimated, I would be happy to FAX or mail this to you.
In other experiments we have sequenced the 5-flanking region of the Pcmt
gene and found an open reading frame corresponding to EST clone CK01830
about 473 bp upstream from the initiation codon of the Pcmt gene.
In my computer search of your site, I found today that Pcmt is in EST
clot 8838 and overlaps with AC013960. Since I was unable to find this
last item or the Pcmt on the scaffold of 3R, I thought this information
could be useful to you. I hope that this not superfluous by now.
As a personal favor, would you acknowledge the receipt of this message?
Sincerely,
Clare O'Connor