Subject: Helping FlyBase: cut-GAL4
Dear Karen,
I think you can help me out here. I am curating a paper for FlyBase
which uses a cut-GAL4 that we have no previous record of. The paper is
Guo et al., 1999
J. Neurosci. 19(19): 8435--8442
and they use a cut-GAL4 for which they thank you. I will make a record
for this transposon/insertion in FlyBase but would like to get a few
details so I can do it accurately. Is it a promoter fusion construct
or is it an enhancer trap insertion of P{GawB}? If it is a promoter
fusion construct perhaps you could give me an idea of how much of the
ct regulatory sequences were used in the fusion, or, if it is an
enhancer trap insertion, please could you tell me whether or not a ct
phenotype is caused and/or whether you know that the insertion lies
within the ct transcription unit. Your reply will be curated as a
personal communication to FlyBase.
Many thanks for your help.
With best wishes,
Rachel.
Subject: ct-GAL4
Dear Rachel,
I've just received the reprint and the description of cut-GAL4 isn't very
informative. However, I know it can't be an enhancer trap line since we
didn't do any enhancer trapping. Since it expresses GAL4 along the wing
margin, I assume it is a GAL4 line we made using the 2.7kb fragment of the
cut locus that had previously been shown to direct wing margin expression
(Jack et al. Development  113:735--747 , 1991).
I hope this is helpful.
Best wishes,
Karen