Subject: FlyBase query
Dear Dr. Fehon,
I am curating your paper for FlyBase:
McCartney et al., 2000, Development 127(6): 1315--1324
and I have a question about the 'P{w+,UpMer+}' construct you used in
the clone experiments.
I would be grateful if you could tell me what promoter is driving Mer
expression - is it the polyubiquitin promoter (I think that's what the
'Up' is for but I wasn't sure)
I look forward to hearing from you,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
>
Subject: Re: FlyBase query
Yes, this is correct. In addition, the polyadenylation signal from the Adh
gene was used at the 3' end.
Thank you for taking care of this.
Rick Fehon
>Dear Dr. Fehon,
>
>I am curating your paper for FlyBase:
>
>McCartney et al., 2000, Development 127(6): 1315--1324
>
>and I have a question about the 'P{w+,UpMer+}' construct you used in
>the clone experiments.
>
>I would be grateful if you could tell me what promoter is driving Mer
>expression - is it the polyubiquitin promoter (I think that's what the
>'Up' is for but I wasn't sure)
>
>I look forward to hearing from you,
>
>Gillian
>
>--------------------------------------------------------------
>Gillian Millburn.
>
>FlyBase (Cambridge),
>--------------------------------------------------------------