Subject: Helping FlyBase: ADRC-279B
Dear Chuck,
We are currently curating the abstracts for the upcoming 41st
(Pittsburgh) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'Preferential transcriptional activation of
Drosophila eukaryotic initiation factor 1A by hop Jak kinase
in larval hemocytes.'
And say 'We previously isolated and sequenced the gene for
Drosophila translation initiation factor D-eIF1A (formerly D-eIF4c)'.
The FlyBase record for this gene is below, and you abstract suggests
that we should rename the gene to be 'eIF-1A'. We should also
presumably change the \*Y lines to read:
\*Y eukaryotic initiation factor 1A
If you could confirm this for me I would be grateful.
\*a eIF-4c
\*z FBgn0026250
\*d cytosolic protein synthesis initiation ; GO:0006440
\*F cytosolic translation initiation factor ; GO:0003744
\*Y eukaryotic initiation factor 4C
\*x FBrf0107670 == Dearolf, 1999.3.5, personal communication
\*x FBrf0106997 == Myrick and Dearolf, 1999, A. Dros. Res. Conf. 40: 487C
\*e Eukaryotic initiation factor 4c
\*i D-eIF4c
\*d cytosolic protein biosynthesis ; GO:0006416
\*F cytosolic translation initiation factor ; GO:0003744
\*Y eukaryotic initiation factor 4C
\*c 90E--91A
\*D Cytological location determined by hybridization to cytologically mapped
\*D P1 clones.
In looking into this I found another gene record that has 'eukaryotic
initiation factor 1A' properties and wonder whether you have anything
to say on whether this record could represent the same gene as eIF-1A
(sadly no cytology in the FBgn0025959 record).
\*a anon- EST:Liang-2.3 
\*z FBgn0025959
\*g AI124295
\*d cytosolic protein synthesis initiation ; GO:0006440 | inferred from sequence similarity
\*F cytosolic translation initiation factor ; GO:0003744 | inferred from sequence similarity
\*Y eukaryotic initiation factor 1A-like
\*j species == Homo sapiens; gene == EIF1A;  GDB:126358 ;  OMIM:603374 
\*j species == Saccharomyces cerevisiae; gene == TIF11;  SGDID:L0002306 
\*j species == Triticum aestivum; gene == 'eIF-1A, translation initiation factor 1A';  SWP:P47815 
\*x FBrf0106226 == Biggin, 1999.2.5, personal communication
\*x FBrf0105875 == Liang and Biggin, 1998, Development 125(22): 4471--4482
\*j species == Homo sapiens; gene == EIF1A;  GDB:126358 ;  OMIM:603374 
\*j species == Saccharomyces cerevisiae; gene == TIF11;  SGDID:L0002306 
\*j species == Triticum aestivum; gene == 'eIF-1A, translation initiation factor 1A';  SWP:P47815 
\*i clone 2.3
\*d cytosolic protein biosynthesis ; GO:0006416 | inferred from sequence similarity
\*F cytosolic translation initiation factor ; GO:0003744 | inferred from sequence similarity
\*Y eukaryotic initiation factor 1A-like
Thank you for your help,
with best wishes,
Rachel.
\----------------------------------------------------------------------
Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
\----------------------------------------------------------------------
Subject: RE: Helping FlyBase: ADRC-347A
requests,
1) StatF is indeed stat92Efrankenstein
2) Yes, eIF-1A is the correct nomenclature, and it is the same gene as that
identified in the paper from Mark Biggin's lab. My post-doc originally
named it eIF-4C (its original name in the mammalian literature, before being
changed to mammalian eIF-1A), but we then changed our nomenclature to
conform with the current name. We have a paper (Myrick and Dearolf) that
just appeared in Gene.
Regards,
Chuck Dearolf
Charles Dearolf, Ph.D.
Associate Professor
Massachusetts General Hospital
Jackson 1402, 55 Fruit St.
Boston, MA 02114