Gene or accession: CG9900
Gene annotation error
Genes CG990, FBgn0029627 and mit(1)15, FBgn0004643 should be merged.
Comments: Sequence alignment of:
1)
AE003424; AAF45794.1
(Celera full length 721aa sequence, strain y; cn bw sp)
2)
X64390; CAB76122.1
(Williams et al, Medline: 92363920, full length 721aa sequence, strain
unidentified)
3)
AL138972; CAB72295.1
(EDGP full length 721aa sequence, strain Oregon-R)
4)
AL121804; CAB65854.1
(EDGP C-terminal 461aa fragment, strain Oregon-R)
demonstrates that CG9900 and mit(1)15 are in fact the same gene, clustal shown
below.
I was concerned that the map position of CG9900 is given as 3A4 and mit(1)15
as 3A9, but looking at the evidence for mit(1)15:
from star coded flat file of genes
\*c 3A9
\*c Left limit from inclusion within Df(1)z-deb81 (FBrf0049883)
\*c Right limit from inclusion within Df(1)z-EM46 (FBrf0054120)
and at the individual Df breakpoint positions, mit(1)15 is actually between
3A1 (Df(1)z-deb81) and 3A9 (Df(1)z-EM46), making 3A4 a possible position.
I hope with this evidence you agree with me,
Regards
Eleanor Whitfield
Swiss-Prot annotator
CLUSTAL W (1.8) multiple sequence alignment
Celera sequence is represented by Swiss-prot accession number: Q9W4X9
CAB72295 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPN
CAB65854 \------------------------------------------------------------
CAB76122 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFMPN
Q9W4X9 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPN
CAB72295 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV
CAB65854 \------------------------------------------------------------
CAB76122 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV
Q9W4X9 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV
CAB72295 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY
CAB65854 \------------------------------------------------------------
CAB76122 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY
Q9W4X9 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY
CAB72295 ETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE
CAB65854 \------------------------------------------------------------
CAB76122 ETIKVKYHVQAYMLQQSLQERFARLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE
Q9W4X9 ETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE
CAB72295 PYNPARLAEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ
CAB65854 \--------------------MRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ
CAB76122 PYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ
Q9W4X9 PYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAQLRPNYKQ
CAB72295 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS
CAB65854 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS
CAB76122 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS
Q9W4X9 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS
CAB72295 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD
CAB65854 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD
CAB76122 TLCEDAAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD
Q9W4X9 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD
CAB72295 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS
CAB65854 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS
CAB76122 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS
Q9W4X9 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS
CAB72295 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT
CAB65854 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT
CAB76122 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT
Q9W4X9 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT
CAB72295 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET
CAB65854 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET
CAB76122 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET
Q9W4X9 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET
CAB72295 VYNATFCELINTFVAELIRRVFTLRHISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL
CAB65854 VYNATFCELINTFVAELIRRVFTLRHISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL
CAB76122 VYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL
Q9W4X9 VYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL
CAB72295 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI
CAB65854 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI
CAB76122 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI
Q9W4X9 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI
CAB72295 V
CAB65854 V
CAB76122 V
Q9W4X9 V
\*
Differences between y; cn bw sp and Oregon-R (AL138972; CAB72295 and AL121804;
CAB65854)
248 248 C \-> A
293 294 QL \-> HV
626 626 D \-> H
Differences between y; cn bw sp and Williams et al (X64390; CAB76122)
58 58 L \-> M
203 203 D \-> A
293 294 QL \-> HV
366 366 V \-> A