Subject: merger of Uba2 and SAE2?
Hello Michael and the Cambridge curators,
Michael, please take a look at the attached ClustalW and BLASTN
alignments for Uba2 and SAE2. There are a few amino acid differences,
but they could be due to sequencing 'differences' (errors and/or
polymorphisms); I don't see evidence for a multigene family (BLAST
hits only one region of one scaffold).
What do you say?
If you want to do a 3-way merger now,
Uba2 FBgn0029113 = CG7528|FBgn0035840|CT23053|
ClustalW of Uba2 and CG7528 is also attached.
Leyla
================================================================================
Uba2 and SAE2 BLASTN alignments
BLAST 2 SEQUENCES RESULTS VERSION BLASTN 2.0.11 Jan-20-2000
\------------------------------------------------------------------------
Sequence 1gi 6934295 Length 2328 (1 .. 2328)
Sequence 2gi 6694273 Length 2352 (1 .. 2352)
 NOTE:The  statistics (bitscore and expect value) is calculated based on the
size of nr database
Score = 4295 bits (2234), Expect = 0.0
Identities = 2282/2305 (99%), Positives = 2282/2305 (99%), Gaps = 2/2305 (0%)
Query: 5
ggcggcgtcacttttctcggcttttccgtatcgcaattttgcaattttctcgccaaaaat 64
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 9
ggcggcgtcacttttctcggcttttccgtatcgcaattttgcaattttctcgccaaaaat 68
Query: 65
aagaaaaaggaaaacgtaaattcagaaaatggcagcagctatcaatggtgttttcccacc 124
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 69
aagaaaaaggaaaacgtaaattcagaaaatggcagcagctatcaatggtgttttcccacc 128
ubiquitin-like protein activati> 1 M A A A
I N G V F P P
Query: 125
cacattgcacgagctggtaaagaagtccaaggtgctggtcgtgggcgccggcggaatcgg 184
|||||||||
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 129
cacattgcaggagctggtaaagaagtccaaggtgctggtcgtgggcgccggcggaatcgg 188
ubiquitin-like protein activati> 12 T L Q E L V K K S K V L V
V G A G G I G
Query: 185
ctgcgaggtgctcaaaaaccttgtgcttagcggctttacagacatcgaaattatcgatct 244
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 189
ctgcgaggtgctcaaaaaccttgtgcttagcggctttacagacatcgaaattatcgatct 248
ubiquitin-like protein activati> 32 C E V L K N L V L S G F T
D I E I I D L
Query: 245
ggacacaattgatcttagcaacttgaaccgacagttcctattccatcgcgagcacgttgg 304
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 249
ggacacaattgatcttagcaacttgaaccgacagttcctattccatcgcgagcacgttgg 308
ubiquitin-like protein activati> 52 D T I D L S N L N R Q F L
F H R E H V G
Query: 305
aaaatctaaggcacgggtggctagggaaagcgcactgagcttcaatccggacgctaagat 364
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309
aaaatctaaggcacgggtggctagggaaagcgcactgagcttcaatccggacgctaagat 368
ubiquitin-like protein activati> 72 K S K A R V A R E S A L S
F N P D A K I
Query: 365
aaccgcttaccacgacagcgtaacatctactgattatggcgtcaacttctttaagaagtt 424
||||||||||||||||||
|||||||||||||||||||||||||||||||||||||||||
Sbjct: 369
aaccgcttaccacgacagtgtaacatctactgattatggcgtcaacttctttaagaagtt 428
ubiquitin-like protein activati> 92 T A Y H D S V T S T D Y G
V N F F K K F
Query: 425
cgatttggtgctcaacgccttggacaacagggccgacaggaatcatgtaaatcgcatgtg 484
|||||||||||||| ||||||||||||||||||||
||||||||||||||||||||||||
Sbjct: 429
cgatttggtgctcagcgccttggacaacagggccgccaggaatcatgtaaatcgcatgtg 488
ubiquitin-like protein activati> 112 D L V L S A L D N R A A R
N H V N R M C
Query: 485
cctcaatgcggatgtcccgctgatcgagagcggcaccgccggctacaatggccaggtgga 544
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 489
cctcaatgcggatgtcccgctgatcgagagcggcaccgccggctacaatggccaggtgga 548
ubiquitin-like protein activati> 132 L N A D V P L I E S G T A
G Y N G Q V E
Query: 545
actgatcaagcgtggactcacccagtgctacgagtgcactccaaaagacaaacagcgaag 604
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 549
actgatcaagcgtggactcacccagtgctacgagtgcactccaaaagacaaacagcgaag 608
ubiquitin-like protein activati> 152 L I K R G L T Q C Y E C T
P K D K Q R S
Query: 605
cttcccgggctgcaccatacgcaatacgccctccgaaccgattcactgcattgtctgggc 664
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 609
cttcccgggctgcaccatacgcaatacgccctccgaaccgattcactgcattgtctgggc 668
ubiquitin-like protein activati> 172 F P G C T I R N T P S E P
I H C I V W A
Query: 665
caagcatctctttaatcaactgtttggtgagtccttggaagatgaggatatttctcctga 724
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 669
caagcatctctttaatcaactgtttggtgagtccttggaagatgaggatatttctcctga 728
ubiquitin-like protein activati> 192 K H L F N Q L F G E S L E
D E D I S P D
Query: 725
tgctgctgatcccgatgcaaaagaaaaagacggcggcgatggaaatggcgagcccaaagg 784
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 729
tgctgctgatcccgatgcaaaagaaaaagacggcggcgatggaaatggcgagcccaaagg 788
ubiquitin-like protein activati> 212 A A D P D A K E K D G G D
G N G E P K G
Query: 785
cgatgggaaggaaaaaggcgaagagtcaaaagaggaaaaggaggccaaggaggatactgc 844
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 789
cgatgggaaggaaaaaggcgaagagtcaaaagaggaaaaggaggccaaggaggatactgc 848
ubiquitin-like protein activati> 232 D G K E K G E E S K E E K
E A K E D T A
Query: 845
caacggcaacataatgcgcattaatactcgccaatgggccaaggactgcaactatgatgc 904
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct: 849
caacggcaacataatgcgcattaatactcgccaatgggccaaggacagcaactatgatgc 908
ubiquitin-like protein activati> 252 N G N I M R I N T R Q W A
K D S N Y D A
Query: 905
gggcaagctgttcaacaagttctttaacgaggacattacctatttgttgcgtatgtcgaa 964
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 909
gggcaagctgttcaacaagttctttaacgaggacattacctatttgttgcgtatgtcgaa 968
ubiquitin-like protein activati> 272 G K L F N K F F N E D I T
Y L L R M S N
Query: 965
tttgtggaagacccgcaaggcacccgtgcccgtgcagtgggataccctgctgcccgaagg 1024
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 969
tttgtggaagacccgcaaggcacccgtgcccgtgcagtgggataccctgctgcccgaagg 1028
ubiquitin-like protein activati> 292 L W K T R K A P V P V Q W
D T L L P E G
Query: 1025
atcctccggtgatcagaaggatgtggccaagcagcatcacaaggtgtggtccatcgagga 1084
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1029
atcctccggtgatcagaaggatgtggccaagcagcatcacaaggtgtggtccatcgagga 1088
ubiquitin-like protein activati> 312 S S G D Q K D V A K Q H H
K V W S I E E
Query: 1085
gtgcgctcaggtctttgccaattcattaaaagagttgagcgctaacttcctgaaactcga 1144
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1089
gtgcgctcaggtctttgccaattcattaaaagagttgagcgctaacttcctgaaactcga 1148
ubiquitin-like protein activati> 332 C A Q V F A N S L K E L S
A N F L K L E
Query: 1145
aggcgacgatactcttgcttgggacaaggacgaccagccagccatggatttcgtggcggc 1204
|||||||||||||||
||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1149
aggcgacgatactctggcttgggacaaggacgaccagccagccatggatttcgtggcggc 1208
ubiquitin-like protein activati> 352 G D D T L A W D K D D Q P
A M D F V A A
Query: 1205
ctgcgccaacgtgcgatcccacattttctacattgagccaaagtcaaggttcgagaataa 1264
|||||||||||||||||||||||||||| |||||||||
||||||||||||||||| |||
Sbjct: 1209
ctgcgccaacgtgcgatcccacattttcgacattgagcgaaagtcaaggttcgagattaa 1268
ubiquitin-like protein activati> 372 C A N V R S H I F D I E R
K S R F E I K
Query: 1265
atcaatggcgggaaacattattcctgctattgccaccacaaatgcgattacggcgggaat 1324
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1269
gtcaatggcgggaaacattattcctgctattgccaccacaaatgcgattacggcgggaat 1328
ubiquitin-like protein activati> 392 S M A G N I I P A I A T T
N A I T A G I
Query: 1325
ttccgtgatgcgggctttcaaagtgctggaggccaagtgggagcagtgccaggccgtcta 1384
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 1329
ttccgtgatgcgggctttcaaagtgctggaggccaagtgggagcagtgcaaggccgtcta 1388
ubiquitin-like protein activati> 412 S V M R A F K V L E A K W
E Q C K A V Y
Query: 1385
tgcccgacttagaccaaatgcacgaaatcacttccttgtaccggacgcttctcttcctgg 1444
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1389
tgcccgacttagaccaaatgcacgaaatcacttccttgtaccggacgcttctcttcctgg 1448
ubiquitin-like protein activati> 432 A R L R P N A R N H F L V
P D A S L P G
Query: 1445
ccccaatcccaattgccatgtatgcgccagcgatccggccattactctcaagatcgatac 1504
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1449
ccccaatcccaattgccatgtatgcgccagcgatccggccattactctcaagatcgatac 1508
ubiquitin-like protein activati> 452 P N P N C H V C A S D P A
I T L K I D T
Query: 1505
gaagcgcatgcgtataaaggagctgcgtgacgaggtcctggttaagacgctcaacatgtt 1564
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1509
gaagcgcatgcgtataaaggagctgcgtgacgaggtcctggttaagacgctcaacatgtt 1568
ubiquitin-like protein activati> 472 K R M R I K E L R D E V L
V K T L N M L
Query: 1565
gaatccggatgtgactgtgcagagcaatggctccattttaatctcctcagaggagggcga 1624
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1569
gaatccggatgtgactgtgcagagcaatggctccattttaatctcctcagaggagggcga 1628
ubiquitin-like protein activati> 492 N P D V T V Q S N G S I L
I S S E E G E
Query: 1625
gaccgaatgcaatgacggcaagctcctaagcgaattaaacattgtggatggtgtgatcct 1684
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1629
gaccgaatgcaatgacggcaagctcctaagcgaattaaacattgtggatggtgtgatcct 1688
ubiquitin-like protein activati> 512 T E C N D G K L L S E L N
I V D G V I L
Query: 1685
caagtgcgacgacttcttccagaactacgagttgagtattatcatttcccacttcgatgc 1744
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1689
caagtgcgacgacttcttccagaactacgagttgagtattatcatttcccacttcgatgc 1748
ubiquitin-like protein activati> 532 K C D D F F Q N Y E L S I
I I S H F D A
Query: 1745
ggagcgcgatgaaaacttgttcgaagtcgtagcagatgcctcacagctgaagccaaagga 1804
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1749
ggagcgcgatgaaaacttgttcgaagtcgtagcagatgcctcacagctgaagccaaagga 1808
ubiquitin-like protein activati> 552 E R D E N L F E V V A D A
S Q L K P K D
Query: 1805
tgaggatcagaaggaggccgtgaaagacaaggaggatgaaccgaaatcggctaagaagcg 1864
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1809
tgaggatcagaaggaggccgtgaaagacaaggaggatgaaccgaaatcggctaagaagcg 1868
ubiquitin-like protein activati> 572 E D Q K E A V K D K E D E
P K S A K K R
Query: 1865
ttctaccaacggggaaggagactcaaaggacgatggaccctccacttcgaagcgcagccg 1924
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1869
ttctaccaacggggaaggagactcaaaggacgatggaccctccacttcgaagcgcagccg 1928
ubiquitin-like protein activati> 592 S T N G E G D S K D D G P
S T S K R S R
Query: 1925
gcccaacgaagtggttgaggaagatgacgatgactgtctggtaatcgaggaagacgaaga 1984
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1929
gcccaacgaagtggttgaggaagatgacgatgactgtctggtaatcgaggaagacgaaga 1988
ubiquitin-like protein activati> 612 P N E V V E E D D D D C L
V I E E D E D
Query: 1985
ccaagcagatgttgttgtcgtggccacagacaagctctctgtgcagagtcccccaaaatc 2044
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1989
ccaagcagatgttgttgtcgtggccacagacaagctctctgtgcagagtcccccaaaatc 2048
ubiquitin-like protein activati> 632 Q A D V V V V A T D K L S
V Q S P P K S
Query: 2045
gggctccaagcgcaagccatgtgaagtaatcgaggatgaggatatcaccgagattttgga 2104
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2049
gggctccaagcgcaagccatgtgaagtaatcgaggatgaggatatcaccgagattttgga 2108
ubiquitin-like protein activati> 652 G S K R K P C E V I E D E
D I T E I L E
Query: 2105
gtcgtctgacgacgaacccgcgggaccaaccaagtgcaaacgttcccgcctggacgattc 2164
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2109
gtcgtctgacgacgaacccgcgggaccaaccaagtgcaaacgttcccgcctggacgattc 2168
ubiquitin-like protein activati> 672 S S D D E P A G P T K C K
R S R L D D S
Query: 2165
gaaccccgtggcagtcatcagtatcgattaatnnnnnnnngaagtttttattcgaataag 2224
||||||||||||||||||||||||||||||||
|| |||||||||||||||||
Sbjct: 2169
gaaccccgtggcagtcatcagtatcgattaataaaaaaaagatgtttttattcgaataag 2228
ubiquitin-like protein activati> 692 N P V A V I S I D ^^^
Query: 2225
ttctcaactgcgcactaaattatgctcttgattccttgtaatttcattcttgatttaaaa 2284
|||||||||||||||||||||||||||||||||||
||||||||||||| ||||||||||
Sbjct: 2229
ttctcaactgcgcactaaattatgctcttgattccctgtaatttcattc-tgatttaaaa 2287
Query: 2285 taaaaacacattttttgtggatgat 2309
|||||||||| ||||||||||||||
Sbjct: 2288 taaaaacaca-tttttgtggatgat 2311
CPU time: 0.11 user secs. 0.06 sys. secs 0.17 total secs. Gapped Lambda K H
1.33 0.621 1.12 Matrix: blastn  matrix:1  \-2 Gap Penalties: Existence: 5,
Extension: 2 Number of Hits to DB: 14 Number of Sequences: 0 Number of
extensions: 14 Number of successful extensions: 3 Number of sequences better
than 10.0: 1 length of query: 2328 length of database: 706,884,452 effective
HSP length: 24 effective length of query: 2304 effective length of database:
699,596,996 effective search space: 1611871478784 effective search space
used: 1611871478784 T: 0 A: 0 X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 1
================================================================================
SAE2 Uba2 CLUSTALW alignment
CLUSTAL W (1.8) Multiple Sequence Alignments
Sequence format is Pearson
Sequence 1: gi|6694274|gb|AAF25197.1|AF193 700 aa
Sequence 2: gi|6934296|gb|AAF31704.1|AF218 700 aa
Start of Pairwise alignments
Aligning...
Sequences ( 1:2 ) Aligned. Score: 98
Start of Multiple Alignment
There are 1 groups
Aligning...
Group 1: Sequences: 2  Score:11481 
Alignment Score 4265
CLUSTAL W (1.8) multiple sequence alignment
gi|6694274|gb|AAF25197.1|AF193
MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50
gi|6934296|gb|AAF31704.1|AF218
MAAAINGVFPPTLHELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50
gi|6694274|gb|AAF25197.1|AF193
LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100
gi|6934296|gb|AAF31704.1|AF218
LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100
gi|6694274|gb|AAF25197.1|AF193
TDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQV 150
gi|6934296|gb|AAF31704.1|AF218
TDYGVNFFKKFDLVLNALDNRADRNHVNRMCLNADVPLIESGTAGYNGQV 150
gi|6694274|gb|AAF25197.1|AF193
ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200
gi|6934296|gb|AAF31704.1|AF218
ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200
gi|6694274|gb|AAF25197.1|AF193
ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250
gi|6934296|gb|AAF31704.1|AF218
ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250
gi|6694274|gb|AAF25197.1|AF193
ANGNIMRINTRQWAKDSNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300
gi|6934296|gb|AAF31704.1|AF218
ANGNIMRINTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300
gi|6694274|gb|AAF25197.1|AF193
PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350
gi|6934296|gb|AAF31704.1|AF218
PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350
gi|6694274|gb|AAF25197.1|AF193
EGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPA 400
gi|6934296|gb|AAF31704.1|AF218
EGDDTLAWDKDDQPAMDFVAACANVRSHIFYIEPKSRFENKSMAGNIIPA 400
gi|6694274|gb|AAF25197.1|AF193
IATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLP 450
gi|6934296|gb|AAF31704.1|AF218
IATTNAITAGISVMRAFKVLEAKWEQCQAVYARLRPNARNHFLVPDASLP 450
gi|6694274|gb|AAF25197.1|AF193
GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500
gi|6934296|gb|AAF31704.1|AF218
GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500
gi|6694274|gb|AAF25197.1|AF193
GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550
gi|6934296|gb|AAF31704.1|AF218
GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550
gi|6694274|gb|AAF25197.1|AF193
AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600
gi|6934296|gb|AAF31704.1|AF218
AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600
gi|6694274|gb|AAF25197.1|AF193
DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650
gi|6934296|gb|AAF31704.1|AF218
DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650
gi|6694274|gb|AAF25197.1|AF193
SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID 700
gi|6934296|gb|AAF31704.1|AF218
SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID 700
================================================================================
Uba2 FBgn0029113= CG7528|FBgn0035840|CT23053|
CLUSTAL W (1.8) Multiple Sequence Alignments
Sequence format is Pearson
Sequence 1: CG7528|FBgn0035840|CT23053|FBa 823 aa
Sequence 2: gi|6694274|gb|AAF25197.1|AF193 700 aa
Start of Pairwise alignments
Aligning...
Sequences ( 1:2 ) Aligned. Score: 99
Start of Multiple Alignment
There are 1 groups
Aligning...
Group 1: Sequences: 2  Score:11503 
Alignment Score 4275
CLUSTAL W (1.8) multiple sequence alignment
CG7528|FBgn0035840|CT23053|FBa
MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50
gi|6694274|gb|AAF25197.1|AF193
MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50
CG7528|FBgn0035840|CT23053|FBa
LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100
gi|6694274|gb|AAF25197.1|AF193
LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100
CG7528|FBgn0035840|CT23053|FBa
TDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQV 150
gi|6694274|gb|AAF25197.1|AF193
TDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQV 150
CG7528|FBgn0035840|CT23053|FBa
ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200
gi|6694274|gb|AAF25197.1|AF193
ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200
CG7528|FBgn0035840|CT23053|FBa
ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250
gi|6694274|gb|AAF25197.1|AF193
ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250
CG7528|FBgn0035840|CT23053|FBa
ANGNIMRINTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300
gi|6694274|gb|AAF25197.1|AF193
ANGNIMRINTRQWAKDSNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300
CG7528|FBgn0035840|CT23053|FBa
PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350
gi|6694274|gb|AAF25197.1|AF193
PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350
CG7528|FBgn0035840|CT23053|FBa
EGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPA 400
gi|6694274|gb|AAF25197.1|AF193
EGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPA 400
CG7528|FBgn0035840|CT23053|FBa
IATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLP 450
gi|6694274|gb|AAF25197.1|AF193
IATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLP 450
CG7528|FBgn0035840|CT23053|FBa
GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500
gi|6694274|gb|AAF25197.1|AF193
GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500
CG7528|FBgn0035840|CT23053|FBa
GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550
gi|6694274|gb|AAF25197.1|AF193
GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550
CG7528|FBgn0035840|CT23053|FBa
AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600
gi|6694274|gb|AAF25197.1|AF193
AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600
CG7528|FBgn0035840|CT23053|FBa
DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650
gi|6694274|gb|AAF25197.1|AF193
DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650
CG7528|FBgn0035840|CT23053|FBa
SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAMSLAS 700
gi|6694274|gb|AAF25197.1|AF193
SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID 700
\*********************************************: .
CG7528|FBgn0035840|CT23053|FBa
RILQQGSRLWNGLSAARGFHLLTRPAAPAIVSIQSSQLVAATTGICQTSG 750
gi|6694274|gb|AAF25197.1|AF193
\--------------------------------------------------
CG7528|FBgn0035840|CT23053|FBa
LLTPGSTLVQQVAGFKVKGRLKRRCKDCYIVVRQERGYVICPTHPRHKQM 800
gi|6694274|gb|AAF25197.1|AF193
\--------------------------------------------------
CG7528|FBgn0035840|CT23053|FBa SMKKRDYKSWILTHATQSKERGY 823
gi|6694274|gb|AAF25197.1|AF193 \-----------------------
================================================================================