Subject: BOK and debcl merge
Dear Cambridge curators,
Please merge BOK and debcl. The BLASTN alignment
of the BOK cDNA (acc. AF216752) and one of the debcl cDNAs
(acc. AF149798) is attached.
(All the debcl cDNAs align with one another.)
Note that within debcl Accession AF222004 is the
statement 'Potential Drosophila homologue of Bcl-2-related
Ovarian Killer (BOK)'.
Also, the BOK CDS is contained within CG12397 (ie
CG12397 has to be split and the intron/exon structure
corrected). BLASTP alignment of the 2 is attached.
Leyla
================================================================================
BOKdebclBLN:
BLAST 2 SEQUENCES RESULTS VERSION BLASTN 2.0.11 Jan-20-2000
\------------------------------------------------------------------------
Sequence 1 gi 6708481 Length 1618 (1 .. 1618)
Sequence 2 gi 6724089 Length 1642 (1 .. 1642)
 NOTE:The  statistics (bitscore and expect value) is calculated based on the
size of nr database
Score = 3009 bits (1565), Expect = 0.0
Identities = 1602/1620 (98%), Positives = 1602/1620 (98%), Gaps = 3/1620 (0%)
Query: 1
gagagggtggtaggccgattccctctccccactgcccgttgaaattcagaatactaagct 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1
gagagggtggtaggccgattccctctccccactgcccgttgaaattcagaatactaagct 60
Query: 61
ctcggttaaacgcggcgaaaaagaaagcaagctctgagcggctgnnnnnnnnntgaagtg 120
||||||||||||||||||||||||||||||||||||||||||||
|||||||
Sbjct: 61
ctcggttaaacgcggcgaaaaagaaagcaagctctgagcggctgaaaaaaaaatgaagtg 120
Query: 121
aaataaaactgggatcgcggcaccagcaacaagttttagtggctcttctttgtgcgtttc 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121
aaataaaactgggatcgcggcaccagcaacaagttttagtggctcttctttgtgcgtttc 180
Query: 181
gttcgtgtttgctgccctgcgctttgctcgccacattcgtcgccgacttttattctgttt 240
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 181
gttcgtgtttgctgccctgcgctttgctcgccacattcgtcgccgacttttattttgttt 240
Query: 241
tgcccattttatcagaatcggagcacctccaaaaaagcccaagacgagctgagcctcagc 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241
tgcccattttatcagaatcggagcacctccaaaaaagcccaagacgagctgagcctcagc 300
Query: 301
tgcgtcgaggtgagctgatccactccgctcccctttcgtgcgctgcccaccgctccccac 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301
tgcgtcgaggtgagctgatccactccgctcccctttcgtgcgctgcccaccgctccccac 360
Query: 361
cgctcacacccgatcccatccaatccaatccgatccgctccgctccgagtgcatagtgca 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361
cgctcacacccgatcccatccaatccaatccgatccgctccgctccgagtgcatagtgca 420
Query: 421
tgcaaagtcgcggggctgggctttcggaattacacaacccacatggggcagcgccaacaa 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 421
tgcaaagtcgcggggctgggctttcggaattacacaacccacatggggcagcgc-aacaa 479
Bcl-2 family member protein 1 M
G Q R N K
Query: 481
aggcctcagcagcaacaatagcgggcagccaagcattgccctgccctcgacttcgaccat 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 480
aggcctcagcagcaacaatagcgggcagccaagcattgccctgccctcgacttcgaccat 539
Bcl-2 family member protein 7 G L S S N N S G Q P S I A L P
S T S T M
Query: 541
ggctcccaccaccagtccgccacccaagctggccaagttcaagtcctcgtcgctggacca 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 540
ggctcccaccaccagtccgccacccaagctggccaagttcaagtcctcgtcgctggacca 599
Bcl-2 family member protein 27 A P T T S P P P K L A K F K S
S S L D H
Query: 601
cgagatctacacggccaatcgccgcggcaccattgccacggcctccagcgactggaaggc 660
||||||||||||||||||||||||||||||||||||
|||||||||||||||||||||||
Sbjct: 600
cgagatctacacggccaatcgccgcggcaccattgcaacggcctccagcgactggaaggc 659
Bcl-2 family member protein 47 E I Y T A N R R G T I A T A S
S D W K A
Query: 661
gctccgcggaggcgtcggtggaggagcaggaggacccggtagcgtacccaatccctctaa 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 660
gctccgcggaggcgtcggtggaggagcaggaggacccggtagcgtacccaatccctctaa 719
Bcl-2 family member protein 67 L R G G V G G G A G G P G S V
P N P S N
Query: 721
cggacgctcccttcacgccggcggacccatgacacgggccgcctccacatcctcgctggc 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 720
cggacgctcccttcacgccggcggacccatgacacgggccgcctccacatcctcgctggc 779
Bcl-2 family member protein 87 G R S L H A G G P M T R A A S
T S S L A
Query: 781
tagcagtacgcgcacgatgactaactaccaggagtacaaaatggatatcatcaaccaggg 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 780
tagcagtacgcgcacgatgactaactaccaggagtacaaaatggatatcatcaaccaggg 839
Bcl-2 family member protein 107 S S T R T M T N Y Q E Y K M D
I I N Q G
Query: 841
gaaatgtctgtgtggtcagtacatcagagcgcggctgcgacgggcaggagtcctcaaccg 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 840
gaaatgtctgtgtggtcagtacatcagagcgcggctgcgacgggcaggagtcctcaaccg 899
Bcl-2 family member protein 127 K C L C G Q Y I R A R L R R A
G V L N R
Query: 901
gaaggtgacacagcgtttgcgcaacatcctggaccccggctcctcgcacgtggtctatga 960
||||||||||||||||||||||||
|||||||||||||||||||||||||||||||||||
Sbjct: 900
gaaggtgacacagcgtttgcgcaatatcctggaccccggctcctcgcacgtggtctatga 959
Bcl-2 family member protein 147 K V T Q R L R N I L D P G S S
H V V Y E
Query: 961
agttttcccggcactgaacagcatgggcgaggaactggagcggatgcacccgcgggtgta 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 960
agttttcccggcactgaacagcatgggcgaggaactggagcggatgcacccgcgggtgta 1019
Bcl-2 family member protein 167 V F P A L N S M G E E L E R M
H P R V Y
Query: 1021
cacaaacatatcgcgacagctgtcgagggccccgtttggcgagctggaggacagcgacat 1080
||||||
|||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1020
cacaaatatatcgcgacagctgtcgagggccccgtttggcgagctggaggacagcgacat 1079
Bcl-2 family member protein 187 T N I S R Q L S R A P F G E L
E D S D M
Query: 1081
ggcgcccatgttgctcaacctagttgccaaggatctttttcgctccagcatcacctgggg 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1080
ggcgcccatgttgctcaacctagttgccaaggatctttttcgctccagcatcacctgggg 1139
Bcl-2 family member protein 207 A P M L L N L V A K D L F R S
S I T W G
Query: 1141
caagataatctcgatatttgccgtatgcggcggctttgccatagactgcgtgcgccaggg 1200
||||||||||||||||||||||||||||||||||||||||
|||||||||||||||||||
Sbjct: 1140
caagataatctcgatatttgccgtatgcggcggctttgccgtagactgcgtgcgccaggg 1199
Bcl-2 family member protein 227 K I I S I F A V C G G F A V D
C V R Q G
Query: 1201
acatttcgactacctacagtgcctgattgacggtctggctgagatcat--aggacgacct 1258
|||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 1200
acatttcgactacctacagtgcctgattgacggtctggctgagatcatagaggacgacct 1259
Bcl-2 family member protein 247 H F D Y L Q C L I D G L A E I
I E D D L
Query: 1259
ggtctactggctgatcgacaacggcggatggttgggcctgtcgcggcacatccgaccccg 1318
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1260
ggtctactggctgatcgacaacggcggatggttgggcctgtcgcggcacatccgaccccg 1319
Bcl-2 family member protein 267 V Y W L I D N G G W L G L S R
H I R P R
Query: 1319
ggtcggcgaatttacgttcttgggatggttgacgctgttcgtgactatctctgcaggcgc 1378
|||||||||||||||||||||||||||||||||||||||
||||||||||||||||||||
Sbjct: 1320
ggtcggcgaatttacgttcttgggatggttgacgctgtttgtgactatctctgcaggcgc 1379
Bcl-2 family member protein 287 V G E F T F L G W L T L F V T
I S A G A
Query: 1379
atatatggtctcaaacgtgtgtcggcgcattggaggtcaactgtattcgctgctgttcta 1438
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1380
atatatggtctcaaacgtgtgtcggcgcattggaggtcaactgtattcgctgctgttcta 1439
Bcl-2 family member protein 307 Y M V S N V C R R I G G Q L Y
S L L F ^^
Query: 1439
gattcgcttgggatcgcgtcgttaagaaatacaatcgtaccatttagtcaatgagagctt 1498
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1440
gattcgcttgggatcgcgtcgttaagaaatacaatcgtaccatttagtcaatgagagctt 1499
Bcl-2 family member protein 326 ^
Query: 1499
caaatcattcctgcttccatgggcaccagtcgtttagtagtatgtaacggaccctgtttt 1558
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1500
caaatcattcctgcttccatgggcaccagtcgtttagtagtatgtaacggaccctgtttt 1559
Query: 1559
acgtataatattgttattccctttctcctctttttgtacatacaaggctattctaggcgc 1618
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1560
acgtataatattgttattccctttctcctctttttgtacatacaaggctattctaggcgc 1619
CPU time: 0.09 user secs. 0.07 sys. secs 0.16 total secs. Gapped Lambda K H
1.33 0.621 1.12 Matrix: blastn  matrix:1  \-2 Gap Penalties: Existence: 5,
Extension: 2 Number of Hits to DB: 5 Number of Sequences: 0 Number of
extensions: 5 Number of successful extensions: 3 Number of sequences better
than 10.0: 1 length of query: 1618 length of database: 706,884,452 effective
HSP length: 24 effective length of query: 1594 effective length of database:
696,399,164 effective search space: 1110060267416 effective search space
used: 1110060267416 T: 0 A: 0 X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 12
(23.8 bits) S2: 19 (37.2 bits)
===============================================================================
BOK is contained within CG12397|FBan0012397|CT26692| (1st 3 exons, 3rd exon to
be extended) (ie CG12397 has to be split)
CG12397 annotation now reads:
AE003789 mRNA join(157782..158619,161030..161173,161229..161606,
161958..161994,162414..163101,163158..163550,
163605..164151,164213..>164266)
/gene='CG12397'
By alignment to mRNA Acc. AF216752, annotation for BOK should be
join(157782..158619,161030..161173,161229..161866)
BLASTP alignment of BOK CDS with CG12397:
>CG12397|FBan0012397|CT26692|FBan0012397  last_updated:000321 
Length = 846
Score = 1232 (433.7 bits), Expect = 7.9e-127, P = 7.9e-127
Identities = 237/237 (100%), Positives = 237/237 (100%)
Query: 1 MAPTTSPPPKLAKFKSSSLDHEIYTANRRGTIATASSDWKALRGGVGGGAGGPGSVPNPS 60
MAPTTSPPPKLAKFKSSSLDHEIYTANRRGTIATASSDWKALRGGVGGGAGGPGSVPNPS
Sbjct: 1 MAPTTSPPPKLAKFKSSSLDHEIYTANRRGTIATASSDWKALRGGVGGGAGGPGSVPNPS 60
Query: 61 NGRSLHAGGPMTRAASTSSLASSTRTMTNYQEYKMDIINQGKCLCGQYIRARLRRAGVLN 120
NGRSLHAGGPMTRAASTSSLASSTRTMTNYQEYKMDIINQGKCLCGQYIRARLRRAGVLN
Sbjct: 61 NGRSLHAGGPMTRAASTSSLASSTRTMTNYQEYKMDIINQGKCLCGQYIRARLRRAGVLN 120
Query: 121 RKVTQRLRNILDPGSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDSD 180
RKVTQRLRNILDPGSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDSD
Sbjct: 121 RKVTQRLRNILDPGSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDSD 180
Query: 181 MAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEII 237
MAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEII
Sbjct: 181 MAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEII 237
================================================================================