Subject: FlyBase query: par-1 Dear Daniel, I am curating your paper for FlyBase: Shulman et al., 2000, Cell 101(4): 377--388 and I have a couple of questions. 1. the lines 'l(2)k05603' and 'EP(2)1144'. which are stated to be in the par-1 locus in the Materials and Methods. My question is, are these two P-element insertions in the 5' UTR of the N1 transcription unit of par-1 ? (in the results it says that 'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 transcription unit' \- so I thought these 2 P-elements might be the other 2 that are not named). Also, do you know whether these P-elements affect the function of both par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' must affect both as we already have it as an allele of mei-W68, but I wanted to check. Is EP(2)1144 viable ? . Gillian \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- > Hi, daniel forwarded your questions to me. >1. the lines 'l(2)k05603' and 'EP(2)1144'. > >which are stated to be in the par-1 locus in the Materials and Methods. > >My question is, are these two P-element insertions in the 5' UTR of the >N1 transcription unit of par-1 ? (in the results it says that >'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 >transcription unit' \- so I thought these 2 P-elements might be the >other 2 that are not named). Your interpretation is correct--all three of these insertions are in the 5'UTR of the N1 transcription unit. I can't recall if this is stated in the paper, but you might want to make note of the fact that the original l(2)k06821 line, which I believe has been discarded public collections, was a double insert line--we recombined off the extraneous insert. > >Also, do you know whether these P-elements affect the function of both >par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' >must affect both as we already have it as an allele of mei-W68, but I >wanted to check. Is EP(2)1144 viable ? l(2)k05603 and EP(2)1144 are both viable, and weak, hypomorphic alleles of both genes. The mei-W68 allelism of 05603 was reported in McKim and Hayashi-Hagihara (1998) and for 1144 the allelism should be cited to Kim McKim (personal communication). . take care, Josh \-- Joshua M Shulman Wellcome/CRC Institute Tennis Court Road Cambridge CB2 1QR England > Subject: FlyBase query: mei-W68 Dear Dr. McKim, I am writing to ask whether it is OK to include in FlyBase some information about 2 P-element insertion lines which I have obtained from Joshua Shulman, but which originally comes from you. I wrote to him about a couple of insertion lines from his paper: Shulman et al., 2000, Cell 101(4): 377--388 the lines are 'l(2)k05603' and 'EP(2)1144' which are in par-1 (and which it turns out also affect mei-W68) I wrote to ask him whether the 2 lines affect the function of both par-1 and mei-W68 or just one of the 2 genes, since the 2 genes share a promoter and 5' UTR. He replied that both lines affect both genes, but said that the information for the allelism of the 2 lines to mei-W68 should be cited as a personal communication from you, and that I should write and check that it it OK with you to include this information in FlyBase. If it is OK with you for this information to be included, then it seems to me that the simplest thing would be to include this information as a personal communication from both you and J. Shulman to FlyBase, I have included below the correspondence between me and Joshua for your information, I look forward to hearing from you, Gillian \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- here is the e-mail correspondence: Subject: FlyBase query: par-1 Dear Daniel, I am curating your paper for FlyBase: Shulman et al., 2000, Cell 101(4): 377--388 and I have a couple of questions. 1. the lines 'l(2)k05603' and 'EP(2)1144'. which are stated to be in the par-1 locus in the Materials and Methods. My question is, are these two P-element insertions in the 5' UTR of the N1 transcription unit of par-1 ? (in the results it says that 'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 transcription unit' \- so I thought these 2 P-elements might be the other 2 that are not named). Also, do you know whether these P-elements affect the function of both par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' must affect both as we already have it as an allele of mei-W68, but I wanted to check. Is EP(2)1144 viable ? Gillian \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- > Hi, daniel forwarded your questions to me. >1. the lines 'l(2)k05603' and 'EP(2)1144'. > >which are stated to be in the par-1 locus in the Materials and Methods. > >My question is, are these two P-element insertions in the 5' UTR of the >N1 transcription unit of par-1 ? (in the results it says that >'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 >transcription unit' \- so I thought these 2 P-elements might be the >other 2 that are not named). Your interpretation is correct--all three of these insertions are in the 5'UTR of the N1 transcription unit. I can't recall if this is stated in the paper, but you might want to make note of the fact that the original l(2)k06821 line, which I believe has been discarded public collections, was a double insert line--we recombined off the extraneous insert. > >Also, do you know whether these P-elements affect the function of both >par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' >must affect both as we already have it as an allele of mei-W68, but I >wanted to check. Is EP(2)1144 viable ? l(2)k05603 and EP(2)1144 are both viable, and weak, hypomorphic alleles of both genes. The mei-W68 allelism of 05603 was reported in McKim and Hayashi-Hagihara (1998) and for 1144 the allelism should be cited to Kim McKim (personal communication). take care, Josh \-- Joshua M Shulman Wellcome/CRC Institute Tennis Court Road Cambridge CB2 1QR England > Subject: Re: FlyBase query: par-1 Dear Josh, thanks for your reply to my questions. I would like to include the information about the various lines in the par-1 locus (the bit below) as a personal communication from you to FlyBase, as it is useful information. Is that OK with you ? Gillian > > >1. the lines 'l(2)k05603' and 'EP(2)1144'. > > > >which are stated to be in the par-1 locus in the Materials and Methods. > > > >My question is, are these two P-element insertions in the 5' UTR of the > >N1 transcription unit of par-1 ? (in the results it says that > >'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 > >transcription unit' \- so I thought these 2 P-elements might be the > >other 2 that are not named). > > Your interpretation is correct--all three of these insertions are in the > 5'UTR of the N1 transcription unit. I can't recall if this is stated in the > paper, but you might want to make note of the fact that the original > l(2)k06821 line, which I believe has been discarded public collections, was > a double insert line--we recombined off the extraneous insert. > > > > >Also, do you know whether these P-elements affect the function of both > >par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' > >must affect both as we already have it as an allele of mei-W68, but I > >wanted to check. Is EP(2)1144 viable ? > > l(2)k05603 and EP(2)1144 are both viable, and weak, hypomorphic alleles of > both genes. The mei-W68 allelism of 05603 was reported in McKim and > Hayashi-Hagihara (1998) and for 1144 the allelism should be cited to Kim > McKim (personal communication). > > Subject: Re: FlyBase query: par-1 Gillian, yes, this is of course, fine. But any reference to the mei-W68 allelism should be cited as personal communication from Kim McKim, you might want to check with him to get his permission directly, but I'm sure he wouldn't have a problem with this (he gave us permission to put all of this in the paper anyway). \--Josh . Subject: Re: FlyBase query: mei-W68 Gillian, In short, your plan sounds like a good way to handle the citation. The two insertions are hypomorphic alleles of mei-W68. Kim _____________________________________ Kim McKim, Ph.D. Waksman Institute, Rutgers University 190 Frelinghuysen RD Piscataway NY 08854 908-445-1164 .
