Gene or accession: AE002935
Assembly error
Comments: AE002935 is a part of PARP gene at 81F on 3R NOT 2R. PARP is
terribly large gene more than 60 kb, and is singl copy gene: PMID: 9565614.
CG17696 gene is exon 1, 2, 3 of PARP. I confirmed the map position on PARP by
PCR. Embryo from Df(3R)10-65/blueBal clossed with Df(3R)4-75/blueBal was
stained with X-gal. Df/Df developed normally until final stage of
embryogenesis. PCR using PARP primers for each exons showed stained embryo,
blueBal/blueBal and blueBal/Df ,have all exons and unstained embryo, Df/Df,
lacks all exons of PARP. DNA extraction was confirmed by PCR using ftz primers
as internal control.
AE002666 have exon 4 of PARP at position 9658...10191 in sense direction. So
that it must be on 3R NOT 2R. Distance between exon 3 and 4 is ~36 kb, so that
there is a ~16 kb gap between AE002935(complement) and AE002666.
AE002892.2 is have PARP gene as notes by BDGP, however predicted start
position is not correct. It is mysterious that both map='41C-41D'
map='81F-81F' are in 'source' of the note. Distance between exon 4 and 5 is
>20 kb, so that there is a >5 kb gap between AE002666 and AE002892.2.