Subject: Re: FlyBase Query (cy1054/5)
>Dear Chihiro,
>
>I made the elavGal4 lines you mentioned by mobilizing the elavGal4
>transgene that was generated by Luo. Luo fused the elav promoter to
>the Gal4 gene. His insertion was on the second and was lethal. I
>did a hop and selected for viable inserts on the 3rd. My lines were
>not generated from C155 and so should not affect the endogenous elav
>gene. I do not know where on the 3rd they have inserted. I
>probably sent Rajagopalan the 3A insert.
>
> Yours, Aaron DiAntonio
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Aaron DiAntonio, M.D., Ph.D.
Assistant Professor
Department of Molecular Biology and Pharmacology
Washington University School of Medicine
Campus Box 8103, 660 S. Euclid
St. Louis, MO 63110
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Subject: FlyBase Query (cy1054/5)
Dear Dr DiAntonio,
I am currently curating two papers for FlyBase:
Simpson et al., 2000, Neuron 28(3): 753--766
Rajagopalan et al., 2000, Neuron 28(3): 767--777
In these papers the authors name you as the source of elav-GAL4 lines.
Simpson et al. state that they were made by mobilising the C155
elav-GAL4. and call them elav-GAL4 3A and 3E, while Rajagopalan only
calls the line used elav-GAL4.
We do not have these in our records \- have they been published before?
If so what name was used to describe them. If they are new can you
confirm that they were made by local hopping the P{GawB} element from
C155 elav-GAL4? Do you have any more details about these lines? (e.g.
are the new insertions within the elav gene? Do the insertions cause
any mutant phenotype? ) Can you also tell me which line you sent to
Rajagopalan et al.?
Any new information will be curated as a personal communication from
you to FlyBase if thats fine with you.
Thanks and best wishes,
Chihiro
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Chihiro Yamada.
FlyBase (Cambridge),
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