Gene or accession: CG7480
Release: 1
Gene annotation error
Gene CG7480 has incorrect exon/intron structure or translation start site.
Comments: Emailed information from Mark Stapleton suggests that CG7480
(l(2)35Aa) is annotated on the wrong strand:
Mark Stapleton wrote:
I looked into the example you mentioned and found a cluster of 11 AT ESTs
using an alignment tool called sim4. Within this cluster was AT01079, which
we have 3' end sequenced. Both the 5' and 3' end reads appear correct in
there orientation with respect to the cloning vector and the 3' read has a
polyA tail. Indeed, by BLASTing against the predicted genes, this clone
appears to be in the opposite orientation. But by using sim4, which aligns
reads to genomic sequence and therefore not against an assumed oriented gene
prediction, the reads are 571bp away from each other and on the opposite
strand from the predicted gene. I can say with some confidence that the
prediction must be misplaced (wrong strand) especially since the cluster of
5' end reads consists of 11 independent clones.
Hope this helps,
Mark
On Thursday 26 April 2001  08:19  am, Henrik Clausen wrote:
> Dear Dr. Stapleton,
>
> We have noticed a cluster of 5' ESTs with apparent reverse orientation.
> All these
> ESTs originate from the same testis library, and we can not find
> information of
> potential 3' sequencing of the clones.
>
> An example is the following:
> >gb|BG634260.1|BG634260 AT30416.5prime AT Drosophila melanogaster adult
>
> testes pOTB7
> Drosophila melanogaster cDNA clone AT30416 5 similar to
> l(2)35Aa: FBan0007480 'enzyme' located on: 2L 35A1-35A1;:
>
> If possible we would very much appreciate help with information you may
> have on
> this library and/or these clones. Thank you in advance for your help, best
> regards,
> Henrik Clausen
> Henrik Clausen, DDS, DSc
> Associate professor