08:07:35  2001
Gene or accession: AE003436 (CG15899 and CG4222)
Release: 2
Gene annotation error
Gene CG15899 and CG4222 has incorrect exon/intron structure or translation
start site.
Comments: 1) Comparison with mammalian Ca2+ channel alpha13 subunit sequences
(NP_06149, NP_066921, NP_066919) suggested that predicted genes CG15899
and CG4222 should instead form a single gene. RT-PCR of D. melanogaster mRNA
(sense primer \- ACTCCTCGCACACCTCGTCA, antisense primer \- GTCGTCCACGCAGGGCTGAT)
confirmed this suspicion. AE003436 nt 217058-211515 constitute an intron. This
intron is of the AT-AC type, just like the corresponding introns in the
mammalian genes. I have not confirmed the putative translation initiation site
of CG15899/CG4222.
2) AE003436 nt 199014-198781 were predicted to form part of an exon. RT-PCR of
D. melanogaster mRNA (sense primer \- GAACCGGCCACCTGCTATGC, antisense primer \-
TCCGGCGGCTGATTATGGTA) showed that they are part of an intron. In other words,
the wrong splice acceptor was predicted.
3) AE003436 nt 180921-180860 were predicted to be an intron. However,
comparison with the mammalian orthologues strongly suggests that these nt are
part of the exon. These and downstream nt (now frameshifted) encode the 4th,
5th and 6th membrane-spanning segments of the 4th homologous repeat domain of
the calcium channel. As yet, I haven't experimentally verified the new
prediction, nor have I identified the stop codon.
>nucleotide
TCCAGCTCGTCCAGTTCGTCCGGCGAACCGAATCTGCCGTATCCGGGATTCCCCGAGATC
TCTATCAGGGCGCTGACGCAGTACACCCGGCCGCGCAACTGGTGCCTGATGCTCATCACC
AATCCATGGTTCGAGCGCGTCTCCATTCTGGTCATCCTGCTCAACTGCATCACTCTCGGC
ATGTATCAGCCCTGCGTGGACGACGCATGTGTGACGAACCGCTGCAAGATCCTTCAGATC
TTCGACGACATCATCTTCGCCTTCTTTGCCCTGGAAATGACCATCAAGATGGTGGCCATG
GGCATCTGTGGCAAGAACACCTATCTGGCAGACTCGTGGAATCGGCTGGACTTCTTCATC
GTTCTGGCAGGTCTGCTGGAGTACGTGATGCATGTGGAGAATCTCAATCTGACTGCCATA
CGAACGATTCGAGTGCTGCGTCCGCTGCGGGCCATCAATCGAATACCCAGCATGCGAATC
CTGGTGATGCTCCTGCTGGACACGCTGCCCATGCTGGGCAATGTCCTGTTGCTCTGCTTT
TTCGTGTTCTTCATCTTCGGAATTATTGGAGTCCAGCTGTGGGAGGGAATTCTGCGACAG
CGATGTAGTCTGATGCGAACTGAAGGCATGGTCTATCCACTCACCCTGTCGCAGTACTAC
GAGTTCTCCAAAGATCAGGACTATATCTGCTCCACGCCAAACGATTCGGGAATGCATCTA
TGCGGAAATTTCCCGCCCTATCGCATTGGTTCGCTGGTCTGTAATGAGGAGGCCAAGCTG
TTCGACTTCAATGAGCCGACGAACACGTCCTGCGTCAATTGGAACCAGTACTATACCACC
TGCAAGCAGAGCGGCGAGAATCCCTTTCAGGGCACGATATCCTTCGACAACATCGGCATG
GCCTGGGTGGCCATATTCCTGGTCATCTCGCTGGAGGGCTGGACGGACATAATGTATTAC
GTGCAGGATGCGCACAGCTTTTGGGATTGGATATACTTTGTGCTGCTGATTGTGATTGGT
TCGTTCTTCATGATCAACCTGTGCCTGGTCGTCATCGCCACGCAATTCTCGGAGACCAAG
AAACGCGAAATGGAGCGGATGCGACAGGAGCGAGCCCGTTATACATCCACTTCAACCCTG
GCCAGCAGCACAAATAACTCGGAACCGGCCACCTGCTATGCGGAGATCGTCAAATATATC
GCCCACCTGTGGCGGCGCTTCAAGAGACGAATGTTAAAGAAGTACAGATTATATAAGTAC
CAGCGACAGCAGCGCAAGGAGGGCCTACTGCCCAATGCCGACAATTTGACCTTTTCGCCG
TCGCGGATCAAGTGCCATCATCCCAAGTGCCCCAAGTACAGTAATCGCAAGCCGTCCAGT
ATTCAGGATCAGATGATTACCGTCATGGTGCCGCTCAATTCGGCTAGCAACAACATTAAT
AATAACAACAACAACAACAGCAACAGTAGCAACCACAATGGCAGTGGAAACCACACAGCT
GCCGGCGGTGGCAACAACAATAACAACAACAACAACAACAATGCCGCCAGCTGCACCACA
GTGGCACTGGTCAATGGGATAAATGGCAGCGCCGCCAGTGTGACCATGTCCTCGGCCCAC
CATCAGCAACATCAATTGCTGCAACATCAACAGCAGCAACACCAAAACCAACAGCAGCAG
CAGCAGCAGCAGCAGCACTCCTCGTCGGACAACACGGAGCAATCTCTGGCCCCAGACGGA
TTGCCCCGGAGTTCCTCGCTGAAAAAATCCACCGCACATCATCAACTGAAGCCGGAGGGC
AGTGCCGCCGAGCAGAAGACCATATTGCTGAAGTTTCCGCAGCAAATGATTGATTCCGAG
CAGCTAATTCTGCAGTTGGGAAATTTGGGCAAGAGTCATCCGTGCACCTCGGGCTTCCTA
AGTCCGCCCACCTCGGCCAGCCGGCGACCTTCGGTGATGTTCAACGAGTACGTGCTGCTC
CACACGCCGCCTGCCCTCAACGCAGATCCGGCAACGGCGGGCACCACCACAACGGTGGCT
CCAACAGTCGCAACAGTTGCCGGCACGGCGGCCGCCGCAGCAGCTGCAGCAGCGACAGGA
TCCGGCAGTGGCAATAACAACCACCAAAATGGCGGTGCAAGTGGAGGAGCTGGAACTGGG
GGAACCACTGAGAAAAGCAACATTTTCTCAACGGAAAAGATGACCCAAGCTGGCGATGGC
AGCATCTGGCAGGTTAATCTGCCGCAGACCATCGGCACCATTGCCAATCCCTATGCCGAT
TGCTCTGAACTTGGTATACACGATGCGATGACTTGTCAAGAGCTCTTAGCATTCTCTGTG
GCCTTTTCAGCGGCATTGCCGACGGGCCAGAGTACGCTAGAGTCATTCTACACATCGCTG
GCCCGCTGCGATCCTCACACCGCAGAGGCGTTGCGGGCGCACCACAAGCCGCGCTCAGTG
CCCACTGGGCAAAATCAAACGACACCGGGCGCGGAAGGAGCCACTGTGATGGTTGCCTCA
ACGGCTGGTGATACCGGCCAGACACTTCAACCCTCGACAGTGTCGGCAGTGGTGGGCGGA
ACCATCGATAGCCATGCGGCGAACCATCGTCGAAAGGAGCACCACCAGCAGTCGCACCAC
CATCATAACAATAACAATACCACCAGTCACAGTCGCAACTATCGCTCACGGCAGGGACAG
GGAAATTCGCGAATGCGTGAGCCACGCGCCCCCACTGGCAACTATATGGAGGACTATGCG
TGCTGTTATGATCTCTACCAGAACGCCCTGTCGCCACTGGACGAACGGCCCAGGCAGAGA
TCGCCGACGACTCGCTGTCTGATCTCCGTGTATCGATGCATGTCGCGCGTGTGCAGCTGG
ATTCGTCGCTACATCCGGCGATTGGTGGAGCACAAGTACTTCCAGCAGGGCATCCTGTTG
GCCATCCTGATTAACACGCTGTCCATGGGCATCGAGTACCATAATCAGCCGCCGGAATTG
ACCGCCATTGTGGAGACGAGCAACGTCGTCTTCTCCGGCATCTTTGCTGTTGAAATGTTA
CTAAAGGTTGTTGCAGAGGGTCCATTCCGCTACATTGCCAATGGATTCAATGTTTTCGAT
GGCATCATCGTTATTCTCAGTGCCATTGAGATCTGTCAGACGTTCATGGGCAACGGAACG
GGTGGTGGTGGCTCCGGACTGTCCGTGTTGCGGACATTCCGGTTGCTGCGAATCCTCAAA
TTGGTCCGGTTTATGCCCAATCTGCGACGCCAGCTATTCGTGATGCTGCGCACAATGGAC
AACGTGGCCGTGTTCTTCTCCCTTCTCGTTTTATTCATCTTTATATTCAGTATACTCGGC
ATGAATCTGTTCGGATGTAAATTCTGCGAGAAAGTTAACGGAGAGATGATTTGCGATCGT
AAGAATTTCGACAGCCTGCTCTGGGCGCTCGTCACAGTGTTCCAGATACTGACGCAAGAG
GATTGGAACGTCGTCCTGTTCAACGGAATGGAAAAGACAAGTCATTGGGCCGCATTGTAC
TTTGTGGCACTAATGACGTTCGGCAATTATGTGCTATTTAATTTATTGGTGGCCATTTTG
GTTGAGGGATTCAGTTCAGAGCGAAATGAACGTCGCGAGCGCGAACAGCGCGAGTTGGTT
AAGAAACTGCGTGAGGAGACGTTGGCCGAGAACTACAGCGATGGTATGTACGATGAGTCG
CGGAGCGAGGCAGACTCCTCGACCACCAACGATAGTTACTACGAGGTGCGCAACCGCTGG
CGATCGGCGGAGGATGTGCGCAAGCTACAGGACTCCGTCGAGCTGATCATCGAGGCCAAG
AGCAACATGCACCGCCAGCGCCTGCTGCAGCCCACCCACGATTACCAGATCAACGAGCTG
CCCGCCTCGTCTGCCGCCCCTTCCGCCTCTGGCACCTCTGGCGCCTCCGCACCTGGCGAC
CGGGAGAGGGACAGGGACAGAGACAGAGATAGGGAGCGAGACAGAGATAGGGACAGGGAG
CGGGACAGAGAGGCGGGCGGCGGCGGCAAGGAGGAGGGGGCGCAGCATGCCAAGCCGCGC
GGCGGCCTCAAAAAGACATACTCCATCAAGGAGCGGCGCAGCGAGGCGCCACGCCTCTCC
AAGATCCGGCTCGCCCGGGATCCGCCCATCATCACGACAACGGCGGCCACGCCGCAGGAC
TCGCCCAGCACCACGTTGGAGCCGGGCATGAGCTTCCGCCAGTGGGGCGACATGGAGCCA
CCCAGTCCGCCCTCTCCGTCGCTTTTGCGACCGCCGAACATCTTTACCGGCGGACAGCGG
AGTCTGGACGAGGGCATACCCTCCATCGATCTCATACCGCCCTCGCCGGTGCTATCCCAC
AAGCCCCTGAATATCCTCAATGCCAGCCAGCTGGGCGTGGGCATGGGAGGCGGTGTCCCC
AGCACCGCCGGCAGTTCGAGCAGCATGCACAGCGTGATCATCGACGACATTTCGAAGAGC
TCCAGCTCAACGGCGGCGGCTACGCCCATTTATGTGCCCACCATCTCGTCCACAGCGGAC
GCGCAGAGCCAGAGTCACAGTCTCAACGATGTAAGTGTGGGCTCCAGTCCAGGAGGTGAG
ATTCCCGCAACGGGAATGAGCAGGAATGCCAACACGGGAGCCTCGACCAGTGGAAGTTCG
TCCAGCGAGCGCCTGCCATTGGCTCCACCTCCTCAGCAGGGGAGTTTTAAGCAGCGCTTA
CGGCGCGGTAGCTCCAAAAAGCGACGTGCCTCCGCTTTGGCCCTGGCCACAGACGACAAT
CCGGCTCGAAGAACGCTGGATAATCAGGCTCGAAGGACCCAGGACGAGGAGGAAGAACAG
CAGCAGCTGAACAATGGCGGCGATAACAGCTGTTTGCTGAGAAACAGCAACGCTGTGGTC
AGCGGTTCCTCCTCCGGCACCAAGGAGACCAATCGCCTGAGTCCACAAAACTCCATACGA
AGGTTATCCAATACACTGAGTATAGGCAGCGGACCGGTGGGCAGTCGTCGGGCATCGGCT
TGCATTTTCAACTCGCAGGTCTATCAGAATCTAAATCAACCGCCCAAACTGCGTCCAGGG
TCCGGGCAACGAAGGATGAGCTCCATCGAACTTGCGTTCAGCAAGACTTCGCACCTGAAT
CTGCACAACTTGGAGGCCAACCGCAAGTCGCTCTCGTATACGAACTCGAAGATGGACCTG
GACAAGTGGAACAAGTCGTACGGCAATCTGAATGAGCCGGACAACATGCTGCAACAGTAC
ATGGAGGCGCGGGACAAGCGCAAGAACTCCATCAGCCACTACAACCTAAAGAAGCGGCTC
GAGGAAAAGGAGCTGCAGCAGCTGCAGCAGCTCCACCAGCAACAGCTGCTCCAGCAACGG
CAGGACTCCTTCTCCTCGACCACCCAGCAGCAGCAGCAGCAGCTGCAGCAGCACCGCTTG
TCCAAGGATCAGCAGCAGCTAGCGATGCAGCCACATTCCATGGTGCCAGGAGGCGGCGAG
CGCTACTCCAAGCTCAAGATGCTCATCGAGCAGCTGACGCCCAAGCACTTTACCACCGAG
CGCGAGGACTACTCGCTCTACATATTCCCAGAGGACAACAGGTTCCGGCAAATCTGTACG
TGGTTTGTCAACCAAAAGTGGTTCGACAACGTGGTCCTGCTGTTCATCGCGCTCAATTGC
ATCACCCTGGCCATGGAAAGACCAAACATCCCTCCAAGCAGCACCGAAAGATTGTTCCTG
GCAACAGCCAATTACGTGTTCACCGTCGTCTTTACCGTCGAAATGTTTATCAAGGTGGTG
GCAACGGGAATGTTTTACGGCCACGACGCCTACTTTACGTCCGGCTGGAATATCATGGAC
GGATCTTTGGTTACCATTTCCATAATTGATTTGTTAATGTCCCTGATTAGCGAATCGAGT
CCGAGGATATTTGGGATTTTAAGGGTGTTTCGACTACTCCGATCCTTGCGGCCGCTGCGG
GTGATCAACCGAGCCCCGGGTCTGAAACTAGTCGTGCAAACGCTCTTATCGTCCCTGCGT
CCCATCGGCAACATCGTGCTGATCTGCTGCACCTTCTTCATCATCTTCGGCATCCTGGGC
GTTCAGCTCTTCAAGGGCACCTTCTACTACTGCGAGGGAGAGAACATCAAGGGCGTGCGG
AATGCGGACGAGTGCCGCAGGATTCCTGGCAACGTTTGGACCAACCGCAAGTACAACTTC
GACGATCTGGGCAAGGCCCTGATGTCTTTATTCGTCCTGAGTTCCCGCGACGGCTGGGTA
AACATCATGTACACCGGCCTGGATGCGGTGGGCGTCGACCAGCAGCCCATCGTTAACTAT
AACGAGTGGCGTCTCCTGTACTTCATCGCCTTCATCCTGTTGGTGGGCTTCTTCGTGCTG
AACATGTTCGTCGGCGTGGTGGTGGAGAACTTCCATCGTTGTCGTGAGGAGCAGGAGAAG
GAGGAGAAGATCCGGCGGGCGGCGAAGAGGGCTCTGCAGATGGAGAAGAAGCGCCGCCGG
ATGCACGAGCCACCTTACTACACCAACTACTCACCCACCCGGATGTTCGTTCACAATGTG
GTGACCTCCAAGTACTTTGATTTAGCCATTGCTGCCGTCATCGGCCTGAACGTGGTCACC
ATGGCCATGGAGTACTACAAGATGCCATCTGGACTCAAGTATGCACTGAAGATCTTCAAT
TACTTTTTTACGGCGGTCTTCATACTGGAGGCCAATATGAAGTTGGTCGCTCTGGGCTGG
AAGCTGTATCTCAAGGACCGCTGGAACCAGCTAGACGTGGGCATTGTCCTGCTCTCGATT
GTGGGCATTGTACTGGAGGAGCTGGAGACGAACACGCACCAGATTATACCCATCAATCCG
ACGATTATACGGGTGATGAGGGTACTGCGGATTGCCAGAGTGCTGAAACTCCTGAAGATG
GCCAATGGCATAAGGGCCCTCCTGGACACCGTGATGCAGGCCCTGCCCCAGGTGGGCAAC
CTGGGACTACTGTTTTTCCTGCTATTCTTCATATTCGCGGCATTGGGCGTGGAGCTTTTC
GGGCGACTCGAGTGCTCCGACGAGATTCCCTGTCAGGGATTGGGCGAGCACGCGCACTTC
GCCAACTTTGGCATGGCTTTCCTCACATTGTTTCGCGTAGCGACTGGCGACAATTGGAAC
GGCATCATGAAGGATACGCTGAGGGATAATTGCGATGACGCGGCCGATTGCGTGCGCAAT
TGCTGCGTCAGCTCGGTTATTGCTCCCATATTCTTTGTGATATTCGTGCTAATGGCGCAA
TTCGTTTTAGTGAACGTCGTCGTGGCTGTACTGATGAAACACCTCGAGGAGAGCCACAAG
CAAATGGAGGACGAGCTCGATATGGAGGTGGAGCTCGAGCGCGAGCTG
>protein
SSSSSSSGEPNLPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLG
MYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFI
VLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCF
FVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYPLTLSQYYEFSKDQDYICSTPNDSGMHL
CGNFPPYRIGSLVCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGM
AWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETK
KREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKY
QRQQRKEGLLPNADNLTFSPSRIKCHHPKCPKYSNRKPSSIQDQMITVMVPLNSASNNIN
NNNNNNSNSSNHNGSGNHTAAGGGNNNNNNNNNNAASCTTVALVNGINGSAASVTMSSAH
HQQHQLLQHQQQQHQNQQQQQQQQQHSSSDNTEQSLAPDGLPRSSSLKKSTAHHQLKPEG
SAAEQKTILLKFPQQMIDSEQLILQLGNLGKSHPCTSGFLSPPTSASRRPSVMFNEYVLL
HTPPALNADPATAGTTTTVAPTVATVAGTAAAAAAAAATGSGSGNNNHQNGGASGGAGTG
GTTEKSNIFSTEKMTQAGDGSIWQVNLPQTIGTIANPYADCSELGIHDAMTCQELLAFSV
AFSAALPTGQSTLESFYTSLARCDPHTAEALRAHHKPRSVPTGQNQTTPGAEGATVMVAS
TAGDTGQTLQPSTVSAVVGGTIDSHAANHRRKEHHQQSHHHHNNNNTTSHSRNYRSRQGQ
GNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSW
IRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEML
LKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILK
LVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKFCEKVNGEMICDR
KNFDSLLWALVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAIL
VEGFSSERNERREREQRELVKKLREETLAENYSDGMYDESRSEADSSTTNDSYYEVRNRW
RSAEDVRKLQDSVELIIEAKSNMHRQRLLQPTHDYQINELPASSAAPSASGTSGASAPGD
RERDRDRDRDRERDRDRDRERDREAGGGGKEEGAQHAKPRGGLKKTYSIKERRSEAPRLS
KIRLARDPPIITTTAATPQDSPSTTLEPGMSFRQWGDMEPPSPPSPSLLRPPNIFTGGQR
SLDEGIPSIDLIPPSPVLSHKPLNILNASQLGVGMGGGVPSTAGSSSSMHSVIIDDISKS
SSSTAAATPIYVPTISSTADAQSQSHSLNDVSVGSSPGGEIPATGMSRNANTGASTSGSS
SSERLPLAPPPQQGSFKQRLRRGSSKKRRASALALATDDNPARRTLDNQARRTQDEEEEQ
QQLNNGGDNSCLLRNSNAVVSGSSSGTKETNRLSPQNSIRRLSNTLSIGSGPVGSRRASA
CIFNSQVYQNLNQPPKLRPGSGQRRMSSIELAFSKTSHLNLHNLEANRKSLSYTNSKMDL
DKWNKSYGNLNEPDNMLQQYMEARDKRKNSISHYNLKKRLEEKELQQLQQLHQQQLLQQR
QDSFSSTTQQQQQQLQQHRLSKDQQQLAMQPHSMVPGGGERYSKLKMLIEQLTPKHFTTE
REDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFL
ATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESS
PRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILG
VQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWV
NIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEK
EEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVT
MAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSI
VGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGN
LGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWN
GIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHK
QMEDELDMEVELEREL