Subject: Re: FlyBase Query (cy1359)
>Hiya David,
>
>I got a quick work question, and a test of your memory/records system.
>I remember that you were pretty thorough with records of your molecular
>biology, so I have faith you'll be able to answer this one.
>
>I'm currently curating:
>
>van Eeden et al., 2001, J. Cell Biol. 154(3): 511--524
>
>In it they use GFPmago17.1, which is one of yours... can you give me
>some molecular details about this and I'll make it a PC from you to
>FB.
>
>Thanks
>
>Chihiro
>
Hi Chihiro,
Hmmm, it isn't using the approved Micklem nomenclature so its a
little difficult to be absolutely sure. Also, most of my notes are
still in Daniel's lab.
I've had a quick look at my thesis \- and as I thought it _definitely_
isn't in there. In fact I have a vague and sneaking suspicion that it
was actually made by Stefan (Grunert).
..
David
\--
__________________________________________________________________
D.R. Micklem
Dept. of Genetics
Cambridge University
__________________________________________________________________
Hi chihiro,
..
I can answer two out of three of your questions, but can't find the details
of GFP-Mago, even in David's thesis. My recollection is that it is in our
standard maternal alpha4 tubulin vector and has GFP fused to the N-terminus
of full-length mago, but you might want to check with David.
..
Dr Daniel St Johnston
Wellcome/CRC Institute
Tennis Court Rd
Cambridge CB2 1QR
United Kingdom