Subject: Re: FlyBase query: gek
Dear Gillian,
Indeed the P-element we used was 9373. I am pretty sure that the
original P-element has a deletion (rather than two P-element
insertion) based on sequencing of the genomic DNA clones 5' to the
P-element. This deletion removes at least the enok gene (please see
our recent publication by Scott et al., Current Biology 11: 99. In
the supplemental material there is also some discussion of the
phenotypes attributed to enok and gek, respectively).
Best wishes,
Liqun Luo
>Dear Dr. Luo,
>
>I am a curator working for FlyBase and I have a question about your
>paper:
>
>Luo et al., 1997, Proc. Natl. Acad. Sci. USA 94(24): 12963--12968
>
>which is about the gek (genghis khan) gene.
>
>In this paper you describe a P-element allele of gek. In the Materials
>and Methods under the 'Molecular Biology' section you say that this
>P-element was 'obtained from the Drosophila Genome Project'. From
>looking at the Genome Project P-elements in this region, I think that
>this P-element must be the 'l(2)09373' line \- as this maps to gek (see
>below)- I would be grateful if you could confirm whether this is the
>case (if I am wrong could you tell me which P-element line it does
>correspond to).
>
>The sequence flanking the 3' end of the P-element in the 'l(2)09373'
>line matches the 5' end of gek, while the sequence flanking the 5' end
>of the P-element maps about 16kb upstream of gek. This either means
>that there has been a deletion associated with the insertion of the
>P-element in this line or there are two P-elements inserted 16kb apart
>in the genome.
>
>In the Materials and Methods you say 'The sequence from the rescued
>plasmid did not match the sequence from nucleotides 1-51 of the gek
>cDNA, or the sequence of the corresponding genomic DNA, and yet the
>clone maps to 60B by chromosome in situ hybridization, indicating that
>a deletion is associated with the P-element insertion'.
>
>(Assuming that the gek allele in your paper is l(2)09373), have you
>sequenced this region of the genome in the gek mutant line \- do you
>know that there is definitely a deletion rather than two P-elements
>inserted close together ?
>
>Any information you give me about this line will be curated as a
>personal communication to FlyBase,
>
>I look forward to hearing from you,
>
>Gillian
>
>--------------------------------------------------------------
>Gillian Millburn.
>
>FlyBase (Cambridge),
>--------------------------------------------------------------