Subject: v(2)k05816 has a P insertion in CG3524 The flanking sequence of v(2)k05816 (Bloomington stock 10580) maps to scaffold AE003581.2 position ~216497. According to the GenBank annotation of this scaffold, this site is in the intron of the CG3524 gene. FlyBase currently lists no mutant alleles of CG3524; CG3524 and v(2)k05816 are listed as separate genes. While it is formally possible that v(2)k05816 is a separate gene from CG3524, located in the intron of v(2)k05816, it seems more likely that v(2)k05816 is an allele of CG3524. A caveat to this interpretation is the possibility of a double insert or a background mutation on the chromosome. \-- \------------------------------------ Robert W. Levis, Ph.D. Carnegie Institution of Washington \------------------------------------