Subject: Helping FlyBase: ADRC-10209
Dear Tripti,
We are currently curating the abstracts for the upcoming 43rd
(San Diego) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'Characterization of cct1 function during oogenesis.'
You mention two genes that are new to FlyBase, cct1 and cct2. Do you know
which of the Genome Project CG annotations your genes correspond to?
All the CGs have corresponding gene records in FlyBase already and we
don't like to make duplicate records for what is actually the same gene
unless we can't avoid it. If your genes do not correspond to CGs then
perhaps you could tell me their map location, as this is valuable
information for the genome annotation project.
I have looked into this and wonder whether cct1 and cct2 correspond to
CG1049 and CG18330? Also, you say 'we have generated mutations in cct1
by excising a P-element inserted in the 5'UTR of the gene'. Would this
P element be P{EP}3346 or P{EP}3379 in CG1049 by any chance? We
already have a record in FlyBase for Cct (FBgn0041342) based on an
earlier abstract from you \- I imagine that this record is a forerunner
to the individual records that I will make for cct1 and cct2, i.e. that
the gene you originally described as Cct is now known to be cct1 and
cct2. If you could confirm this for me (or otherwise set me straight)
I would be most grateful.
Thank you very much for your help,
with best wishes,
Rachel.
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Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
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Subject: Re: Helping FlyBase: ADRC-10209
Dear Rachel,
Your analysis of the cct genes is correct--cct1 corresponds to CG1049
and cct2 corresponds to CG18330. The gene that I previously called 'cct',
I am now calling 'cct1'. At the time, I didn't realize that there was a
second homolog. We have made P-element excisions, but used an insertion
line that was generated in our lab (called BN81). We have not used any of
the EP lines for this purpose.
Best regards,
Tripti