Subject: Re: FDlyBase Query (cy1667)
Dear Chihiro
Thank you for your message about the CtBGal4 \- here is something about
it.........
The CtB-Gal4 line refers to a promoter fusion construct that we have
generated. The construct contains a 3.3kb promoter fragment from the cut
gene (Jack & DeLotto, 1995) fused to the GAL4 coding sequence and inserted
into pCaSpER. In this line, Gal4 is expressed in the embryonic Malpighian
tubules throughout development and is maintained into larval life. This
line also drives low levels of expression in the foregut.
Jack & DeLotto (1995) Genetics 139: 1689-1700
Please let me know if you need any further information.
best wishes helen
Helen Skaer
Department of Zoology
University of Cambridge
Downing Street
Cambridge CB2 3EJ
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Subject: FDlyBase Query (cy1667)
Dear Helen,
I am currently curating your paper for FlyBase:
Sudarsan et al., 2002, Development 129(4): 935--944
I have a quick question I was hoping you could answer for me,
In your materials and methods you mention a previously unpublished Gal4
line that you call CtB-Gal4.
Can you give me some information about this line?
Is is an enhancer trap or a promoter fusion? If it is the former can
you tell me which transposon was used to generate this line (e.g.
P{GawB}). If the latter can you give me some molecular details about
tyhe transposon? Does the CtB stand for the gene C-terminal Binding
Protein?
Any new information you give me will be curated as a personal
communication from you to FlyBase, if thats fine with you.
Thanks
Chihiro
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Chihiro Yamada.
FlyBase (Cambridge)
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