Isolation and characterization of Df(2R)BSC22
Megan Deal-Herr and Kevin Cook
Bloomington Stock Center, Indiana University
Df(2R)BSC22 was isolated as a P transposase-induced male recombination event involving P{EP}betaTub56DEP2640 and P{PZ}mus20902448. The deletion was isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 females X cn1 P{PZ}mus20902448/P{EP}betaTub56DEP2640 bw1 sp1; TMS, Sb1 P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints 56D7-E3;56F9-12. Df(2R)BSC22 failed to complement 18wDelta7-35 and P{lacW}l(2)k00705k00705.