Subject: FlyBase Query (cy1739) Dear Dr O'Connor, I am currently curating your paper for FlyBase: Adachi-Yamada and O'Connor, 2002, Dev. Biol. 251(1): 74--90 I have some questions I was hoping you could answer for me. .. UAS-sal \------- salmScer\UAS.cdCa de Celis et al., 1996, Nature 381(6581): 421--424 Lunde et al., 1998, Development 125(21): 4145--4154 de Celis et al., 1999, Development 126(12): 2653--2662 Expression of a BamHI-KpnI salm cDNA fragment containing the full open reading frame is driven by Scer\UAS Scer\GAL4 binding sites. salmScer\UAS.cKa Bradley and Andrew, 2001, Development 128(15): 3001--3015 Chen et al., 1998, Development 125(24): 4959--4968 Elstob et al., 2001, Development 128(5): 723--732 Kuhnlein and Schuh, 1996, Development 122(7): 2215--2223 Expression of salm fragment from nucleotide 370 to nucleotide 5555 of the composite cDNA sequence (Kuhnlein et al., 1994, EMBO J. 13(1): 168--179) is governed by Scer\UAS regulatory sequences. .. Subject: Re: Reply to query about our paper Hi Chihiro, Mike O'Connor told me that the UAS-sal we used was derived from Sean Carroll in Univ. Wisconsin. It was also labelled with '43A'. The insertion is on the 3rd chromosome. These are all we know about the UAS-sal line. Best regards, Takashi .. Hi Chihiro, Sorry for the delay of my response. As I told you, the UAS-sal43A is not our original. So I would like you to inquire to Sean Carroll .. about recording it as a new allele in the FlyBase. Thanks in advance, Takashi .. <up>FlyBase curator comment. Email sent to Sean Carroll. Kathy Vaccaro replied on his behalf</up> Subject: uas-sal line Dear Chihiro Yamada, I checked our fly stock list and the line w;Cyo/+;UAS-sal 43A/TM2 has listed for a source as Rosa Barrio. .. That is all the information I have. Hope it helps.