Gene or accession: CG4154
Release: 3
cDNA or EST error
Gene cDNA sequence:
>correct splice variant 2
ATGTACGGACTGCTGCTGGAGAACCTCTCCGAGTACATCAAGTCCGTTTACGGCGAGGAGAAATGGGAGGACATCCGGCG
ACAGGCTGGCATCGATTCGCCCTCCTTCAGCGTCCATCAGGTCTATCCCGAGAATCTGCTGCAGAAGCTGGCGAAAAAGG
CTCAACAGGTTCTGGGCGTTTCTGAGCGGGATTTCATGGACCAGATGGGCGTCTACTTCGTGGGCTTTGTGGGCCAGTAT
GGCTACGATCGTGTCCTTTCCGTCCTGGGCCGCCACATGCGCGACTTTCTCAACGGACTGGACAACCTGCATGAGTACCT
GAAGTTCTCGTATCCGCGGATGAGAGCTCCCAGCTTTATCTGCGAAAACGAAACGAAGCAGGGTCTGACCCTGCACTATC
GTTCCAAGCGTCGAGGATTCGTCTACTACACGATGGGTCAAATCCGAGAGGTGGCACGGTATTTCTATCACAAGGAGATG
CATATTGAGCTCGTTCGGGAAGAGATCCTCTTTGACACGGTCCATGTGACCTTCCAACTGACCTTCGATAATCGGGCCTT
CACACTTGCATCCTTGGCCATGACCCGGGAGGAGAAACATCTGCCCATCAGTGCTCATGTGCTGTTTGAGATATTTCCCT
TTTGCATGGTTTTTGGTGCTGATATGGTTGTGCGTAGTATTGGTAACTCTTTGATGGTGATTTTACCGGAACTTCTGGGA
AAAAAAATTACGGCCTGGTTCGATCTAGTTCGTCCCTTAATTGCATTCAAGTTTCAGACTATACTCAATCGTACTAACAA
CATTTTCGAGCTGGTCACTGTGGATCCAGTTACGGAGAGATTCGATGTGCAAAACGAGGACTTACTGCAGCATGAAGATG
GCAGCGAACCGGAAAAGTCTCTTAGATTAAAAGGTCAAATGGTTTACATGGAGAACTGGCGCATGATTATGTTTCTGGGT
ACTCCCGTAATGCCGGATCTGACCTCACTAATAACCACAGGTCTATATATCAACGATCTGTCCATGCACGACTTCAGCAG
AGATCTCATGCTGGCGGGCACTCAACAATCGGTTGAACTGAAGTTGGCACTGGATCAAGAACAGCAGAAGTCAAAGAAGC
TAGAGGAGTCCATGAGATTGTTGGATGAGGAGATGCGTAGAACGGATGAGCTGCTGTATCAGATGATACCCAAACAGGTG
GCTGATAGGCTCCGACGGGGCGAGAATCCCATTGATACCTGTGAGATGTTCGATAGCGTTTCCATCCTGTTCTCAGACAT
CGTTACCTTCACCGAGATTTGCAGCCGCATTACTCCGATGGAGGTAGTGTCCATGCTGAACGCTATGTACTCCATTTTCG
ATAAGCTGACGGAGCGGAATTCAGTGTACAAGGTGGAAACTATTGGTGATGCGTATATGGTGGTGGCGGGTGCTCCGGAT
AAGGATGCCAATCACGCCGAGCGAGTCTGTGATATGGCCCTGGATATGGTGGATGCGATAACCGATCTAAAGGATCCCTC
AACGGGCCAGCACTTGAGGATACGTGTGGGCGTCCACTCGGGCGCAGTGGTGGCGGGCATCGTGGGCCTTAAGATGCCGC
GTTACTGTCTCTTTGGCGATACGGTCAATACCGCCTCACGAATGGAGTCCACCAGCATCGCCATGAAGGTGCACATATCC
GAGTCTACGAAGGTTCTTATTGGACCCAACTACAAGATCATCGAGCGCGGTGAGATCGATGTAAAGGGCAAGGGCACCAT
GGGCACATACTGGCTGGAGGAACGAGAGAACCGGCTGCCTCTGCAACTAACCGCTGCTCTGCAAGTTCATCCGCTCTCAC
CTGTCCCACCGACGCCCACACCCAAGACGAAGGCCATCATGCCTCCTGTATCCAAGCCGTTGACTCCGATGATGCCCGTT
TCAGTTTCCCTGGCAGCTTCCATGCCCACCAGCAACGTTCCTGCTGTGGATGTGATGGCTTCATCATCCAGCATTTCCGG
TTTGGCACTAACAGCCGCTGCAGCGGCCCACATGTCGCTACATCACCAGGCGGTGGTGGCGGAGGCATTAACTGGAGCTT
CAGTTGAAGTGGCACTACCATCAGTAGCTTCTGGCGCAACAGGAGCGGCAGCGGGTGGAGGTGCACCGTCGGATGACCGC
AACAGTCGCATCTACTCCCCCGTGACCTTCAAGGATGTGGCTCGACGAAGCGTAGCCAATTCTCCGGTAAGGAGCTGTGC
TCAACCGGATCAGGAGAGACGTCGCGAGTCCCGCTCCAATTCCACGGGCCACGTGTTTATGCGAACTCCTTCGGAAATTT
TCGGCTCTCTCATCCTGGACACCGAGGAGTTTCTCGAGGATCTGCAAATCTCACGTTCCTCCCTGGCAAACAATAACAAC
AATCAGAGCCCCTGCGGTTTTAGCCCAACTCCTCCATTCCGGATTGGCAGCGCACCGCCCAAGCCGAGACCTAGTAATCC
GGATAAATTTACACCGGAGGAACTGGCCGCCATGGATCAACTGACGCCTCCGTCAACGGCACCAGCCAGAGAAACGGCCA
GCTGCAGCAGTGCATCATTGGATCGCGACAAGGCGACCAAGCTGAAAAAAATCACTTTCTCCAACAGCAGCAGTCTGGAT
GCAACCACTCCAACTGCGTTGGCTGCTGTGGTATGTCCAATGAGAACCAAGTCACCGCCAATGGCAGTGGCTCCAGTGAT
GCACATGGTACAGGCTAGCACCAGCAAAGGCGACGGCCAACGACCAGGCTCGAAGGATTCAGTATCATCCATTTCCCTGC
ACTCACCTCCTCCGCATCGATCTAACTCGGCACCAGCCAGGTGA
Protein sequence:
>correct protein variant 2
MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQY
GYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEM
HIELVREEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLG
KKITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLG
TPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQV
ADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPD
KDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHIS
ESTKVLIGPNYKIIERGEIDVKGKGTMGTYWLEERENRLPLQLTAALQVHPLSPVPPTPTPKTKAIMPPVSKPLTPMMPV
SVSLAASMPTSNVPAVDVMASSSSISGLALTAAAAAHMSLHHQAVVAEALTGASVEVALPSVASGATGAAAGGGAPSDDR
NSRIYSPVTFKDVARRSVANSPVRSCAQPDQERRRESRSNSTGHVFMRTPSEIFGSLILDTEEFLEDLQISRSSLANNNN
NQSPCGFSPTPPFRIGSAPPKPRPSNPDKFTPEELAAMDQLTPPSTAPARETASCSSASLDRDKATKLKKITFSNSSSLD
ATTPTALAAVVCPMRTKSPPMAVAPVMHMVQASTSKGDGQRPGSKDSVSSISLHSPPPHRSNSAPAR.
Comments: I believe I sent you a slightly erroneous sequence for splice
variant 2. The exon-intron structure is the same. The error is a point
mutation (unique to the clone) I failed to catch and fix in the sequence I
sent you. It is at position 1984 in the nucleotide sequence: TCACCATCCA
should be TCATCATCCA. I also found a difference in all of my clones compared
to the genomic sequence from flybase. At position 657 in the nucleotide
sequence your genome says: TTTTTGGGGCT and all of my clones say: TTTTTGGTGCT.
This does not change the amino acid. In the previous splice variant 2
sequence I sent, I fixed that sequence to read as your genomic. In the
current corrected sequence I put it back to TTTTTGGTGCT since it is like this
in all my clones. So to sum up, there are two changes in this new sequence I
am sending you. There should be one difference in the protein sequence.
Terribly sorry for this little mess and please E-mail me for further
clarification if needed!
. Thank you very much!
\-Kristofor