FlyBase Gene Report: Dmel\Gyc88E

General Information

Symbol

Dmel\Gyc88E

Species

D. melanogaster

Name

Guanylyl cyclase at 88E

Annotation Symbol

CG4154

Feature Type

protein_coding_gene

FlyBase ID

FBgn0038295

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

guanylate cyclase (4.6.1.2)

Gene Summary

Guanylyl cyclase at 88E (Gyc88E) encodes an atypical soluble guanylyl cyclase. It forms heterodimers with the products of Gyc89Da or Gyc89Db to form an oxygen-sensitive soluble guanylyl cyclase, which likely functions as a neuronal oxygen detector, activated at reduced oxygen levels. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Gyc-88E

Key Links

Genomic Location

Cytogenetic map

88E2-88E2

Sequence location

3R:15,150,375..15,191,242 [+]

Recombination map

3-56

RefSeq locus

NT_033777 REGION:15150375..15191242

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (11 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

enables carbon monoxide binding

inferred from direct assay

(Huang et al., 2007)

enables enzyme binding

inferred from physical interaction with UniProtKB:Q9VEU5

(Morton, 2004)

inferred from physical interaction with UniProtKB:Q9VEU6

(Morton, 2004)

enables GTPase activity

inferred from direct assay

(Morton, 2004)

enables guanylate cyclase activity

inferred from direct assay

(Huang et al., 2007, Morton et al., 2005, Langlais et al., 2004)

inferred from direct assay

(Morton, 2004)

enables heme binding

inferred from direct assay

(Huang et al., 2007)

enables nitric oxide binding

inferred from direct assay

(Huang et al., 2007)

enables oxygen binding

inferred from direct assay

(Huang et al., 2007)

enables oxygen sensor activity

inferred from direct assay

(Huang et al., 2007)

enables protein heterodimerization activity

inferred from physical interaction with UniProtKB:Q9VEU5

(Langlais et al., 2004)

enables protein homodimerization activity

inferred from physical interaction with FLYBASE:Gyc88E; FB:FBgn0038295

(Huang et al., 2007)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables guanylate cyclase activity

inferred from biological aspect of ancestor with PANTHER:PTN002902243

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR011645

(InterPro Project Members, 2004-)

enables heme binding

inferred from electronic annotation with InterPro:IPR011644, InterPro:IPR038158

(InterPro Project Members, 2004-)

enables nitric oxide binding

inferred from biological aspect of ancestor with PANTHER:PTN002902310

(GO Reference Genome Project, 2011-)

enables oxygen sensor activity

inferred from biological aspect of ancestor with PANTHER:PTN002902310

(GO Reference Genome Project, 2011-)

enables phosphorus-oxygen lyase activity

inferred from electronic annotation with InterPro:IPR018297

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in nitric oxide-cGMP-mediated signaling

inferred from direct assay

(Morton, 2004)

involved_in response to hypoxia

inferred from mutant phenotype

(Vermehren-Schmaedick et al., 2010)

involved_in response to reactive oxygen species

inferred from direct assay

(Morton, 2004)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in cGMP biosynthetic process

inferred from electronic annotation with InterPro:IPR011645

(InterPro Project Members, 2004-)

involved_in cyclic nucleotide biosynthetic process

inferred from electronic annotation with InterPro:IPR001054, InterPro:IPR018297

(InterPro Project Members, 2004-)

involved_in intracellular signal transduction

inferred from electronic annotation with InterPro:IPR001054, InterPro:IPR018297

(InterPro Project Members, 2004-)

involved_in nitric oxide-cGMP-mediated signaling

inferred from biological aspect of ancestor with PANTHER:PTN002276750

(GO Reference Genome Project, 2011-)

involved_in response to oxygen levels

inferred from biological aspect of ancestor with PANTHER:PTN002902243

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of guanylate cyclase complex, soluble

inferred from direct assay

(Huang et al., 2007, Morton et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of guanylate cyclase complex, soluble

inferred from biological aspect of ancestor with PANTHER:PTN002902243

(GO Reference Genome Project, 2011-)

inferred by curator from GO:0004383

(Langlais et al., 2004, Morton, 2004)

Gene Group (FlyBase)

SOLUBLE GUANYLATE CYCLASES ATYPICAL

UNCLASSIFIED GTPASES

Protein Family (UniProt)

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. (Q8INF0)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

guanylate cyclase activity

GTP = 3',5'-cyclic GMP + diphosphate (4.6.1.2)

RHEA 13665: GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Gene Group (FlyBase)

SOLUBLE GUANYLATE CYCLASES ATYPICAL -: Atypical soluble guanylate cyclases are enzymes that synthesize cyclic GMP. These enzymes are activated in response to hypoxia, however they are only slightly activated by or are insensitive to nitric oxide. (Adapted from FBrf0179844).
UNCLASSIFIED GTPASES -: This group comprises GTPases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

Heterodimers with Gyc-89Da and Gyc-89Db are activated in response to changing oxygen concentrations, alerting flies to hypoxic environments. Under normal oxygen concentrations, oxygen binds to the heme group and results in low levels of guanylyl cyclase activity. When exposed to reduced oxygen concentrations, the oxygen dissociates from the heme group resulting in activation of the enzyme.

(UniProt, Q8INF0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gyc88E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8INF0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gyc88E-RD

FBtr0110961

NM_001043246

4824

1097

Gyc88E-RE

FBtr0306245

NM_001260186

4049

1097

Gyc88E-RF

FBtr0334926

NM_169616

4028

1090

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Gyc88E-PD

FBpp0110261

121.2

1097

7.29

FBpp0297355

121.2

1097

7.29

FBpp0306946

120.4

1090

7.14

NP_731974

AAF55135

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1097 aa isoforms: Gyc88E-PD, Gyc88E-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer, and heterodimer; with Gyc-89Db and Gyc-89Da, in the presence of magnesium or manganese.

(UniProt, Q8INF0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q8INF0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gyc88E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

larval ventral nerve cord | segmentally repeated

(Langlais et al., 2004)

embryonic brain

(Langlais et al., 2004)

embryonic stage 17

peripheral nervous system | segmentally repeated

(Langlais et al., 2004)

neuron of basiconic sensillum | subset

(Langlais et al., 2004)

neuron of external sensory organ | subset

(Langlais et al., 2004)

third instar larval stage

brain

(Langlais et al., 2004)

larval ventral nerve cord

(Langlais et al., 2004)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

organism

(Langlais et al., 2004)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

organism

Comment: both Gyc88E-RA and Gyc88E-RC transcripts

(Langlais et al., 2004)

Additional Descriptive Data

Gyc88E transcript is expressed at a higher level in adults than in larvae. Both Gyc88E-RA (Gyc-88E-S) and Gyc88E-RC (Gyc-88E-L) are expressed at both stages. Embryonic expression is seen the brain, and in a segmentally repeated pattern in the ventral nerve cord starting at stage 15 or 16. Expression is observed in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segmentsa; embryonic PNS expression colocalizes with that of Gyc-89Db. In the third instar larva, expression is observed in scattered cells throughout the brain, but most prominently in a small cluster of cells in the anterior medial region of each brain lobe. Larval expression in the ventral ganglion is observed in scattered cells located laterally and at the midline.

(Langlais et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of guanylate cyclase complex, soluble

inferred from direct assay

(Huang et al., 2007, Morton et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Gyc88E in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gyc88E

View exonic expression by tissue for Dmel\Gyc88E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41825/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0038295

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0038295

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0038295

Flygut - An atlas of the Drosophila adult midgut

FBgn0038295

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gyc88E

Transgenic constructs containing regulatory region of Gyc88E

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gyc88E^GD11230

viable, with Scer\GAL4^Gyc89Da.1.9

Gyc88E^GD11230

viable, with Scer\GAL4^Gyc89Db.3.4

Gyc88E^GD11230

viable, with Scer\GAL4^Mef2.PR

Gyc88E^GD11230

viable, with Scer\GAL4^pnr-MD237

Gyc88E^GD11230

viable, with Scer\GAL4^tin.CΔ4

Gyc88E^GD11230

Other Phenotypes

Allele

abnormal stress response | third instar larval stage

abnormal stress response | third instar larval stage, with Scer\GAL4^Gyc89Da.1.9

Gyc88E^GD11230

abnormal stress response | third instar larval stage, with Scer\GAL4^Gyc89Db.3.4

Gyc88E^GD11230

abnormal stress response | third instar larval stage (with Gyc88E^S451F)

Gyc88E^V474M

abnormal stress response | third instar larval stage (with Gyc88E^V474M)

Gyc88E^S451F

abnormal taste perception, with Scer\GAL4^Gyc89Da.1.9

Gyc88E^GD11230

abnormal taste perception, with Scer\GAL4^Gyc89Db.3.4

Gyc88E^GD11230

increased cell number | larval stage

Gyc88E^V474M

increased occurrence of cell division | larval stage

Gyc88E^V474M

increased size | larval stage

Gyc88E^V474M

Phenotype manifest in

Allele

embryonic/larval optic lobe | larval stage

Gyc88E^V474M

larval brain

Gyc88E^V474M

larval central brain

Gyc88E^V474M

larval neuroblast | increased number

Gyc88E^V474M

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (23)

Hsap\GUCY1A1

3 of 14

Yes

Hsap\GUCY1A2

3 of 14

Yes

Hsap\GUCY1B1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (23)

Rnor\Gucy1b2

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (24)

Mmus\Gucy1b2

5 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\gucy1b2

4 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\grn

1 of 13

Yes

Xtro\gucy2d

1 of 13

Xtro\LOC100496629

1 of 13

Yes

Danio rerio (Zebrafish) (30)

Drer\gucy1b2

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Drer\si:ch211-132f19.7

2 of 14

Drer\si:dkey-37g12.1

2 of 14

Drer\si:dkey-206f10.1

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\npr2

1 of 14

Drer\npr3

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (39)

Cele\gcy-31

11 of 14

Yes

Cele\gcy-36

4 of 14

Cele\gcy-32

3 of 14

Cele\gcy-33

3 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (25)

Agam\AgaP_AGAP001985

12 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP009087

1 of 12

Agam\AgaP_AGAP012805

1 of 12

Agam\AgaP_AGAP012965

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CYR1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cyr1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Gyc88E. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

5 of 13

5 of 13

5 of 13

5 of 13

Ac3

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

EHR

2 of 13

Gucy2d

2 of 13

CG14877

1 of 13

Phlpp

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GUCY1A1; guanylate cyclase 1 soluble subunit alpha 1

model of Moyamoya disease

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GUCY1A1; guanylate cyclase 1 soluble subunit alpha 1

3 of 14

139396

MOYAMOYA DISEASE 6 WITH OR WITHOUT ACHALASIA; MYMY6

Moyamoya disease

GUCY1A2; guanylate cyclase 1 soluble subunit alpha 2

3 of 14

601244

GUCY1B1; guanylate cyclase 1 soluble subunit beta 1

3 of 14

139397

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Gyc88E

molecular sieving, molecular weight estimation by staining

(Huang et al., 2007)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer, and heterodimer; with Gyc-89Db and Gyc-89Da, in the presence of magnesium or manganese.

(UniProt, Q8INF0 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0038295

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-56

Cytogenetic map

88E2-88E2

Sequence location

3R:15,150,375..15,191,242 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88E2-88E2

Limits computationally determined from genome sequence between P{EP}CG33967^EP666 and P{lacW}Hsc70-4^L3929

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-56

(FlyBase, 2016)

3-55.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

93114

w^*; P{UAS-Gyc88E.V}2

28608

y¹ v¹; P{TRiP.HM05096}attP2

37419

y¹ w^*; Mi{MIC}Mf^MI04259

63353

w¹¹¹⁸; PBac{IT.GAL4}Gyc88E^0490-G4

53208

y¹ w^*; Mi{MIC}Gyc88E^MI09996

53210

y¹ w^*; Mi{MIC}Gyc88E^MI10009 Mf^MI10009

34476

y¹ w^*; Mi{MIC}Mf^MI02345/TM3, Sb¹ Ser¹

35075

y¹ w^*; Mi{MIC}Mf^MI01975/TM3, Sb¹ Ser¹

52184

y¹ w^*; Mi{MIC}Gyc88E^MI09619/TM3, Sb¹ Ser¹

62736

w¹¹¹⁸; PBac{IT.GAL4}Gyc88E^0249-G4 Mf^0249-G4/TM6B, Tb¹

VDRC

v101672

P{KK105392}VIE-260B

v21798

w¹¹¹⁸; P{GD11230}v21798

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0038295

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

SD17859

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0038295

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Gyc-89Db shows no guanylyl cyclase activity when expressed in COS-7 cells in the absence of additional subunits, in the presence of either Mg²⁺ or Mn²⁺ cofactor. However, when Gyc-89Db and Gyc88E are co-expressed in COS-7 cells, the level of guanylyl cyclase activity, in the presence of either Mg²⁺ or Mn²⁺, is higher than when Gyc88E is expressed alone.

(Langlais et al., 2004)

COS-7 cells co-expressing Gyc88E and Gyc-89Da or Gyc88E and Gyc-89Db accumulate low levels of cGMP under normal atmospheric oxygen conditions and are potently activated under anoxic conditions, suggesting that the Gyc88E/Gyc-89Da and Gyc88E/Gyc-89Db protein heterodimers may act as oxygen sensors.

(Morton, 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Source for identity of Gyc88E CG4154 was sequence comparison ( date:031005 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Gyc88E CG4154

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG4154

(Biswas et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Langlais, 2002.7.30, Langlais, 2002.7.31)

GYC

(Morrison et al., 2000)

Gyc-88E

(Vermehren-Schmaedick et al., 2011, Derbyshire et al., 2010, Vermehren-Schmaedick et al., 2010, Huang et al., 2007, Morton and Vermehren, 2007, Langlais et al., 2004, Morton, 2004, Langlais, 2003.8.28)

Gyc88E

(Fang et al., 2026, Li et al., 2023, Xu et al., 2023, Qiu et al., 2022, Pang et al., 2021, Schiffman et al., 2020, Clandinin and Owens, 2016-, Kwon et al., 2013)

Name Synonyms

Guanylyl cyclase at 88E

(Langlais et al., 2004)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41825

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8INF0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8INF0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8INF0

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41825/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0038295

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0038295

FlyMine - An integrated database for Drosophila genomics

FBgn0038295

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4154

MARRVEL_MODEL - MARRVEL (model organism gene)

41825

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0038295

DroID - A comprehensive database of gene and protein interactions.

FBgn0038295

DRSC - Results frm RNAi screens

FBgn0038295

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0038295

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0038295

Flygut - An atlas of the Drosophila adult midgut

FBgn0038295

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008423

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

References (49)

The following external sites may use different assemblies or annotations than FlyBase.

-0.67

Report Sections